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Phaeohelotium monticola?
Maren Kamke,
21-10-2015 06:12
Hi everybody,the very large apothecia (up to 9 mm) grow on soil (maybe grass-roots) in a wet meadow and are smooth. There are three different kinds of textura, the excipulum is of textura angularis/globulosa and textura prismatica, the medulla of textura intricata with oil-drops. The asci are clavate, biseriate with 8 aseptate spores. I measured 122-129x11-12 µm, with croziers. The reaction with Barals solution is negative even after KOH treatment. The ascospores are straight to curved, with oil-amount about 3 or 4, and measure about (11-17) 14,56 x 4,54 (4-5,5) µm. The paraphyses are filled with refractive oil-drops which don't dissappear with KOH treatment.
Regards, Maren
Hans-Otto Baral,
21-10-2015 11:05
Re : Phaeohelotium monticola?
Hi Maren
The colour is exactly that of monticola. Lacking iodine reaction is no problem.
But I cannot believe that the drops in the paraphyses of pic 10 don't disappear in KOH. Also the drops in the medulla I think should disappear (they might be SCBs?) though they are indeed very refractive.
The colour is exactly that of monticola. Lacking iodine reaction is no problem.
But I cannot believe that the drops in the paraphyses of pic 10 don't disappear in KOH. Also the drops in the medulla I think should disappear (they might be SCBs?) though they are indeed very refractive.
Maren Kamke,
21-10-2015 17:26
Re : Phaeohelotium monticola?
Hi Zotto,
thank you very much.
I checked the reaction of the paraphyses again. The first picture is in water, the second in KOH 3% the third in KOH+Baralscher Lösung. The oil-drops become nonrefractive but they are still there, I think, because they could be stained in IKl.
The drops in the medulla disappear indeed, look at pictures four and five.
Regards, Maren
PS: Was bedeutet SCB?
thank you very much.
I checked the reaction of the paraphyses again. The first picture is in water, the second in KOH 3% the third in KOH+Baralscher Lösung. The oil-drops become nonrefractive but they are still there, I think, because they could be stained in IKl.
The drops in the medulla disappear indeed, look at pictures four and five.
Regards, Maren
PS: Was bedeutet SCB?
Hans-Otto Baral,
21-10-2015 17:44
Re : Phaeohelotium monticola?
Yes, so these are SCBs in the medulla. You could test CRB which will not staind them, but the VBs in the paraphyses. In your KOH image they are indeed visible but distorted, usually you see nothing in KOH. Also there is usually not stain anymore in IKI after KOH.
Strange is also that you observed a prismatica. I assume it was at the margin. This is more typical of P. epiphyllum, but there the apos are never so yellow, and the paraphyses are filled with smaller VBs (multiguttulate).
Strange is also that you observed a prismatica. I assume it was at the margin. This is more typical of P. epiphyllum, but there the apos are never so yellow, and the paraphyses are filled with smaller VBs (multiguttulate).
Martin Bemmann,
21-10-2015 21:21
Re : Phaeohelotium monticola?
Dear Zotto,
I am also ignorant what SCBs are (sub cutical bodies? or what? ;-) ).
I can't remeber to come accross this acronym.
Regards
Martin
I am also ignorant what SCBs are (sub cutical bodies? or what? ;-) ).
I can't remeber to come accross this acronym.
Regards
Martin
Raúl Tena Lahoz,
21-10-2015 22:01
Re : Phaeohelotium monticola?
KOH-soluble Cytoplasmic Bodies (SCBs)... ;-)
Martin Bemmann,
21-10-2015 22:05
Re : Phaeohelotium monticola?
OK S for soluble...
Thanks
Martin
Thanks
Martin
Maren Kamke,
23-10-2015 05:56
Re : Phaeohelotium monticola?
Hi Zotto,
I checked on the excipulum again, the images show the flank of the apothecium from top to bottom. It can be seen that the upper flank is of textura prismatica, ca. from the middle downward it changes to textura angularis. The last picture shows the medulla in BKS.
Regards, Maren
Thank you, Raúl for the enlightenment :).
I checked on the excipulum again, the images show the flank of the apothecium from top to bottom. It can be seen that the upper flank is of textura prismatica, ca. from the middle downward it changes to textura angularis. The last picture shows the medulla in BKS.
Regards, Maren
Thank you, Raúl for the enlightenment :).
Hans-Otto Baral,
23-10-2015 10:20
Re : Phaeohelotium monticola?
This is indeed interesting! I thought that the excipulum wa sused as a main feature to separate epiphyllum when studying herbarium material. Strange is that the two species are genetically very distinct, as far as we presently know.
Your CRB pic looks like a faint stain?
Your CRB pic looks like a faint stain?
Raúl Tena Lahoz,
23-10-2015 10:24
Re : Phaeohelotium monticola?
Yes, I think there's some kind of reaction, so then those vacuoles are VBs.
Raúl
Raúl