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Ombrophiloid helotiale on Betula leaves
Michel Hairaud, 09-06-2015 15:26
Michel Hairaud

Bonjour à tous,


Voici une récolte d'hélotiale sur feuilles de bouleau en zone de tourbière pour laquelle je ne trouve pas d'identité. Merci de vos remarques.  


Here is an ombrophiloid helotiale  collection on Betula leaves in peat-bogs . Any help will be appreciated


 


Apos : up to 0.5 cm, nearly translucent white at first , then greyish , rather fleshy , wrapped in a gelatinous gangue at first.


Ascus 105-115 (125) x 9-10,5, IKI -, H+, Spores 9,5-11,5 (13) x 4,5-5


Paraphyses x 2µm with few small VBs


No cristals seen


Ombrophila translucent would suit the substrate but doesn't match several major caracters such as IKI negative reaction.


Amitiés. Michel

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Hans-Otto Baral, 09-06-2015 15:31
Hans-Otto Baral
Re : Ombrophiloid helotiale on Betula leaves
Hi Michel

I don't have an idea... "Mollisia" spectabilis seems excluded too.

Zotto
Nicolas VAN VOOREN, 09-06-2015 15:44
Nicolas VAN VOOREN
Re : Ombrophiloid helotiale on Betula leaves
Et du côté des Pezoloma "non poilus"...
Guy Garcia, 09-06-2015 18:11
Re : Ombrophiloid helotiale on Betula leaves
Salut Michel,

Bien que la longueur sporale moins longue, ta collection doit être proche des récoltes citées sur ce même forum à ce lien
http://www.ascofrance.fr/search_forum/19272

Amitiés, Guy
brigitte capoen, 09-06-2015 18:15
Re : Ombrophiloid helotiale on Betula leaves
Bonjour Michel, Zotto et Nicolas,

  Je viens de faire une recherche dans  la révision des British Helotiaceae de Dennis et je trouve page 164 dans le texte concernant Ombrophila obstricta mention de O. umbonata (Pers.) Karst. avec des spores de la dimension qui correspondent 7-12 x 3-5 µm

Ombrophila umbonata (Pers.) P. Karst., Bidr. Känn. Finl. Nat. Folk 19: 89 (1871) f. umbonata, dont le nom actuel serait Niptera umbonata (Pers.) Fuckel, Jb. nassau. Ver. Naturk. 23-24: 292 (1870) [1869-70] (Index Fungorum)


 


Je viens de trouver ceci: http://ascofrance.fr/forum/24425/une-idee


 


Pezoloma ou Ombrophila obstricta


 


Amitiés


Brigitte

Hans-Otto Baral, 09-06-2015 20:10
Hans-Otto Baral
Re : Ombrophiloid helotiale on Betula leaves
Yes Nicolas and Guy, and indeed Brigitte, I finally came to the same result.

I am not sure if Michel's apothecia have that thick external gel layer of thin hyphae on the excipulum? O your section, Michel., I am not sure. it should be obvious, at least at the lower flanks.

Zotto
Michel Hairaud, 10-06-2015 00:47
Michel Hairaud
Re : Ombrophiloid helotiale on Betula leaves

Quel magnifique forum que cet Ascofrance ! Merci à chacun d'entre vous pour vos réponses  


My fungus was collected at 30 km from Jean Louis Jalla's collecting site(http://ascofrance.fr/forum/24425/une-idee) and on the very same substrate (I found it in two sites actually and each time on Betula leaves ). It is noticeable that I also found larger spores in more mature apos (11-13 µm long versus 9,5-10,5 in others and both ''types'' with free (thus mature) spores  measurements)   


Yes Zotto, as seen on macro pics, a gel sheath wrapps the fresh apos , it leaves a thin membrane also on the hymenium , coloured in CRB .  I admit it's not so readable on the section pic .


AMitiés Michel


 


 

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Hans-Otto Baral, 10-06-2015 08:37
Hans-Otto Baral
Re : Ombrophiloid helotiale on Betula leaves
Hi Michel and others

So when this fungus might be restricted to Betula leaves then I am sure the following sample by Ruben Martinez Gil is also the same:

http://www.ascofrance.fr/forum?page=136

Spores of 10.5 to 13.5 x 4.5-5.5 microns fit very well, and his images show that they may but must nt contain a bigger drop at each end.

I would very much like you to send a portion of this sample for sequencing to Danny Haelewaters in Massachusetts. He might presently be on holidays as I got no response in another matter. I will tell you when he is back.

Zotto
Michel Hairaud, 10-06-2015 09:42
Michel Hairaud
Re : Ombrophiloid helotiale on Betula leaves

Yes Zotto, good idea.


Ruben's post on Ascofrance  is http://www.ascofrance.fr/search_forum/29832


It would also be useful to compare with Enrique's find which I think different (spores much longer and differently shaped ) and Guy Garcia's which I think closer .


Amitiés


Michel

Hans-Otto Baral, 10-06-2015 10:05
Hans-Otto Baral
Re : Ombrophiloid helotiale on Betula leaves
Do you mean Guy Garcia's sample od 30-07-2006 on leaf debris? Yes, habitat and micros look quite close.

Guy Garcia's find on soil with rootlets (VII.2012) has spores with the bigger oil drops away from the poles, perhaps this has some meaning. Also spores are larger.


Enrique's sample Pezoloma, 20.VII.2012-1.jpg has much too long spores.