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18-03-2009 17:43

NC NC

Buenas tardes: A ver qué os parece este ascomice

16-03-2009 23:09

Yannick Mourgues Yannick Mourgues

Bonsoir à tous. Voici une chose curieuse trouv

15-03-2009 09:41

Alain GARDIENNET Alain GARDIENNET

Bonjour, un pyréno sur ilex. qui me pose probl

14-03-2009 00:20

Roland Labbé

Voici une planche d'un Discomycète blanc inconnu.

13-03-2009 21:24

Jean Pierre Dechaume Jean Pierre Dechaume

Sur conseils de Rémy Péan, je vous soumets quelq

11-03-2009 22:48

Hans-Otto Baral Hans-Otto Baral

Hi I have here a pyreno with unitunicate asci t

11-03-2009 22:30

Yannick Mourgues Yannick Mourgues

Diaporthe arctii Bonsoir. Je profite que l'on

11-03-2009 16:58

Guy Garcia

Est-ce que ça cadre pour Lentomitella cirrhosa ?

11-03-2009 00:00

Miguel Ãngel Ribes Miguel Ángel Ribes

Buenas noches Tengo varias recolecciones de Lac

10-03-2009 18:59

Alain GARDIENNET Alain GARDIENNET

Bonsoir, Avant même d'avoir fait la micro, je v

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2014-02-07-Scutellinia
Lepista Zacarias, 08-02-2015 16:42
I would like to have your help to identify this Scutellinia, of which I attach some data below.
Thank you in advance,
zaca

Data:
A few apothecia growing on soil under shrubs;
Spores:
(13.8) 16.1 - 18.8 (19.7) x (10) 11.5 - 13.8 (15.1) µm
Q = (1.3) 1.33 - 1.49 (1.5) ; N = 50
Me = 17.4 x 12.4 µm ; Qe = 1.4
Asci: 220-260 x 12-16 µm;
Paraphysis tips globose up to 10 µm in diameter;
Hairs up to 350 x 32 µm.

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Beñat Jeannerot, 09-02-2015 06:44
Beñat Jeannerot
Re : 2014-02-07-Scutellinia
Adio Zaca,

How are you ?
For this collection, impossible to say without spores in BC...
Probably around nigrohirtula complex, but spores seem to be not mature...

Beñat
Lepista Zacarias, 09-02-2015 11:55
Re : 2014-02-07-Scutellinia
Hi Beñat,
I hope everything is right with you as well.

I suppose that by BC you mean "Bleu de Crésyl". I posted some photos of it in the 4th group; The stain was not very effective; Maybe I have to let the blue act for a litle longer. Anyway, I will do it again (in the last apothecia I have, the collection was really short) and post here the results.

Best regards,
zaca
Malcolm Greaves, 09-02-2015 16:06
Malcolm  Greaves
Re : 2014-02-07-Scutellinia
Zaca
The usual stain for Scutellinia spores is Lactophenol Cotton Blue.
Mal
Lepista Zacarias, 10-02-2015 22:14
Re : 2014-02-07-Scutellinia
Thanks, Malcolm, for the warning.
But, I experienced the same problem with Lactophenol Blue than before with Crésyl Blue, the stain is poor and is unevenly distributed. I upload the result of this try.
Regards, 
zaca
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Chris Yeates, 11-02-2015 02:12
Chris Yeates
Re : 2014-02-07-Scutellinia
Hi Zaca
but as Beñat has pointed out the spores are immature and therefore cannot be of any help. See if you can make a spore drop - with a (thin) marker pen draw a small circle on a microscope slide; turn the slide over and place the hymenial surface of the Scutellinia directly over the circle.
If you leave that for a good period of time you can check the dry microscope slide for ejected (mature) spores in the area within the circle under the compound microscope at x100 or x200; if you see spores, mount in Cotton Blue in Lactophenol, heat gently and show the results.

I hope Beñat does not disagree too much with this procedure I often use . . . .

amitiés
Chris
Lepista Zacarias, 12-02-2015 12:14
Re : 2014-02-07-Scutellinia
Thanks, Chris,
for your detailed explanation that certainly will be helpful in future.
Unfortunately this time it didn´t work: in fact, after an entire day I was not able to see any spore. The material was already very scarse.
Regards,
zaca