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Hello.A very tiny pyrenomycete sprouting sparsely
asco bleu à grande spore (mais immature)
M Jonathan,
23-11-2014 21:09
récolté en chambre humide, (sur du bois peu pourri) un bel ascomycete bleu, la majorité des éléments était encore fortement immature, mais certaine asque présentait des spores fortement immature (dimension 35-40*7-10). trop jeune peut être pour être identifié, mais il me semble distinctif. beaucoup de granule sur les paraphyse et celle-ci sont faiblement ramifié. https://farm8.staticflickr.com/7506/15242215623_b8fd989e88_b.jpg
Jonathan M
Hans-Otto Baral,
23-11-2014 21:22
Re : asco bleu à grande spore (mais immature)
Hi Jonathan
this looks to me to be a Patellaria close to P. atrata. I distinguish a P. crassispora nom. prov. which has a blue pigment and in which the slightly wider spores are much less tapered towards base and tend to be multiguttulate.
Zotto
this looks to me to be a Patellaria close to P. atrata. I distinguish a P. crassispora nom. prov. which has a blue pigment and in which the slightly wider spores are much less tapered towards base and tend to be multiguttulate.
Zotto
M Jonathan,
23-11-2014 21:29
Re : asco bleu à grande spore (mais immature)
thank you, P atrata was one of my suspected specie, but i though it was supposed to be almost pure black, but time will tell, they might get mature in the damp chamber, so i will be able to confirm if this is indeed P crassipes.
Hans-Otto Baral,
23-11-2014 22:55
Re : asco bleu à grande spore (mais immature)
Yes, try maturation! P. atrata has an olivaceous pigment around the tips of paraphyses whereas P. crassispora a bluish.
M Jonathan,
24-11-2014 01:49
Re : asco bleu à grande spore (mais immature)
from this point, how long do you think it should take for it to be fully mature?
Hans-Otto Baral,
24-11-2014 09:37
Re : asco bleu à grande spore (mais immature)
This is difficult. usually these fungi are most of their time dry. When you keep them prmanently moist it can be that they get overgwon by faster-growing fungi. Patellaria withstands many weeks of compete dryness. How fast they mature I do not know, but max. 1 week should be enough to get an immature ascus mature I think.
M Jonathan,
27-11-2014 15:40
Re : asco bleu à grande spore (mais immature)
i found some mature one on the same piece of wood, it a shame that those picture do not show how much blue the paraphyse tip actually are, if you want i dry it and ask someone to make culture of them and then sequence it.
spore 50-53*10-12
https://farm8.staticflickr.com/7544/15702336837_87832d0f03_b.jpg
https://farm8.staticflickr.com/7570/15702063909_6bc8535317_b.jpg
https://farm9.staticflickr.com/8583/15886160211_75dc54c393_b.jpg
https://farm9.staticflickr.com/8583/15886160211_75dc54c393_b.jpg
in situ, the blueish tint is faint but clear
spore 50-53*10-12
https://farm8.staticflickr.com/7544/15702336837_87832d0f03_b.jpg
https://farm8.staticflickr.com/7570/15702063909_6bc8535317_b.jpg
https://farm9.staticflickr.com/8583/15886160211_75dc54c393_b.jpg
https://farm9.staticflickr.com/8583/15886160211_75dc54c393_b.jpg
in situ, the blueish tint is faint but clear
Hans-Otto Baral,
27-11-2014 18:24
Re : asco bleu à grande spore (mais immature)
Yes, I know this photography problem with colours....
If you have the possibility for a culture and sequence this would be fine . Long ago we cultured P. atrata and P. andina, and they both formed a coelomycetous anamorph (unreported in the literature to our knowledge).
Sequences are only a few in GenBank about Patellaria, so this would be valuable in any case!
Zotto
If you have the possibility for a culture and sequence this would be fine . Long ago we cultured P. atrata and P. andina, and they both formed a coelomycetous anamorph (unreported in the literature to our knowledge).
Sequences are only a few in GenBank about Patellaria, so this would be valuable in any case!
Zotto
M Jonathan,
27-11-2014 18:36
Re : asco bleu à grande spore (mais immature)
thank you! i try my best with a quite cheap microscope!
do you know what media was used for the cultures? i will start to dry them today, but it will probably take a few week before i can go to the laboratory again
regards.
do you know what media was used for the cultures? i will start to dry them today, but it will probably take a few week before i can go to the laboratory again
regards.
Hans-Otto Baral,
27-11-2014 20:27
Re : asco bleu à grande spore (mais immature)
The species will easily survive some weeks. The menia we used were usually CMA or MEA, often only half of the normal concentration. I think this species is rather easily to culture.
Zotto
Zotto