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Pale orange, hairy "Trichonectria"?
Björn Wergen, 28-10-2012 09:54
Björn Wergenhello everybody,

this is the second time I have found this small fungus growing on old Digitalis purpurea stems, the first one I have seen this was in Karawankental, Austria, in May 2012. Now it is the end of October and it seems as if this species grows the whole year.

Well, just look at the photos, I have compared the two collections and they show obviously the same species. But what is it? I have tried keys to Trichonectria and Lasionectria, but everytime I thought I got it, there had been differences in spore size. Perithecia are about 0,4-0,7 mm, Wall 15-22µm, the whole surface of the perithecia has single hyaline hairs with a thickened base and 1-2 septa.  

Arr I am angry about this, the species is so clearly and characteristic, but I am not able to identify it :(

Perhaps you can help me. The brown spores you see in the last photo perhaps do not belong to the "Nectria", I did not see them in the first collection.

regards,
björn

edit: I forgot the hairs: 40-50x3,5-5µm.
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Alain GARDIENNET, 28-10-2012 10:20
Alain GARDIENNET
Re : Pale orange, hairy "Trichonectria"?
Hi Björn,
Very nice fungus.
Perhaps a Lasionectria closed to L. sylvana ? It seems to be different, as you said.
Are your ascospores striated ? 
Alain
Björn Wergen, 28-10-2012 13:06
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi Alain,

I did not see any striate ascospores, but perhaps I should try it again with CB. I will also try Lugol reaction.

regards
björn
Christian Lechat, 28-10-2012 17:30
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Hi Björn,

I think it is Lasionectria genus but it would be useful to see a vertical section of the ascomatal wall. Are you sure of the size of perithecia? they seem very large...

Christian
Björn Wergen, 29-10-2012 22:53
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi Christian, hi Alain,

here are some new photos of the wall. It seems to be 18-23µm thick and consists of flattened cells. I had also seen that the surface of the spores is striated :)

Finally I have meassured the perithecia. They are 0,15-0,35 mm.

regards,
björn
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Christian Lechat, 30-10-2012 05:39
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
unfortunately, the section is not enough legible, could you try to do it less thick? You should try with dried material. I know that it is difficult.

Have a nice day,
Christian
Alain GARDIENNET, 30-10-2012 07:09
Alain GARDIENNET
Re : Pale orange, hairy "Trichonectria"?

Christian, what do you think about sylvana ? Ascospores are a bit too long, aren't they ?
Is it possible for this species or could it be another sp. ?
Alain  

Christian Lechat, 30-10-2012 08:30
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Salut Alain,
L. sylvana is not like this, hairs are very different in size (up to 200µm long) and fasciculate
Christian?
Björn Wergen, 30-10-2012 09:24
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Dried material? The material is dried now but it seems to be impossible. I will try it this evening when I come back home :)

regards,
björn
Björn Wergen, 09-11-2012 20:38
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
New photos :)
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Christian Lechat, 10-11-2012 04:32
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Hi Björn,
your images show the ascomatal wall in top view without section, it would be better to make a vertical section as on the attached photo ( Lasionectria sp.). You can make such section with a razor blade under a dissecting microscope. Don't worry, I'm sure that you are able to do it.


All my best,


Christian

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Björn Wergen, 10-11-2012 07:44
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi Christian,

the problem is, that I have only a x15 microscope, with this it seems to be impossible to make such a section. I have to buy a real dissecting microscope...

I will do so and will answer when I get some new photos :)

regards and thanks a lot,
björn
Christian Lechat, 10-11-2012 08:32
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
OK Björn,
I understand,

Christian
Björn Wergen, 23-11-2012 21:22
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi again,

now I am able to make some better sections of the Lasionectria :)

Are they good enough Christian? Or can I do something better?
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Björn Wergen, 23-11-2012 21:53
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Some more photos of the wall
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Christian Lechat, 24-11-2012 06:39
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Hi Björn,
nice section!
So, I think that it is Lasionectria but there is no known species which fit with your specimen.
Are the striations dehiscing from ascospore when slightly crushed under the cover slip? ?
Jacques and I have a Lasionectria sp. nov. (in press) from FWI, collected on Passiflora which would fit with yours, but it would be necessary to compare them.
Could you please to send me a part of your collection?

Christian
Björn Wergen, 24-11-2012 10:22
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi Christian,

of course I can send you something from the collection.  

regards,
björn
Christian Lechat, 24-11-2012 11:26
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Thank you Björn,
I would be surprised if your specimen was the same species than mine but we have to check it.

All the best,
Christian
Björn Wergen, 24-11-2012 16:19
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
If it is not the same as yours, what do we do then? Describe it as new in the same article as your one? :D
Christian Lechat, 24-11-2012 18:21
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Hi Björn,
our publication is in press, we cannot intervene any more.
I don't believe that it is the same species

Let's wait to see,

Christian
Christian Lechat, 08-12-2012 17:20
Christian Lechat
Re : Pale orange, hairy "Trichonectria"?
Dear Björn, ?
I received your specimen today, I studied it and I think that it is a new species of Lasionectria.
I cultivated it today, as soon as I will have its anamorph and molecular data, I will contact you in private.

Cheers,
Christian
Björn Wergen, 09-12-2012 02:02
Björn Wergen
Re : Pale orange, hairy "Trichonectria"?
Hi Christian,

many thanks for this, it is great. And I had this species twice, one new record for Austria and one also for Germany :D

regards,
björn