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Bernard CLESSE
Pourriez-vous me confirmer ma détermination de ce
Bonjour !
Voici une planche et une photo macro d'un Asco qui nous est inconnu. Il a été récolté à Lac Beauport dans la région de Québec.
Nous croyons que ce pourrait être Mollisia ligni.
Voici les données :
Date de récolte : 06 / 07 / 2009
Substrat : tige d'herbacée creuse d'un terrain vague
Ascome : 0,3-0,6 mm de diam.
Sporée non disponible
Spores étroitement et longuement ellipsoïdes, légèrement plus effilées à une extrémité, parfois très légèrement arquées, lisses, non septées, avec une petite guttule foncée à chaque pôle, 6-9 x 1,5-2 µm, 7,1 x 1,9 µm en moyenne (10 spores), Q = 3,74
Asques à 8 spores bisériées, avec crochet à la base (rare) et appareil apical amyloïde, 27-40 x 5-7 µm
Paraphyses cylindriques, un peu élargies à l'apex, à 2-3 septa, doublement ramifiées au tiers inférieur, à contenu huileux, 30-36 x 3-4 µm, dépassant les asques de 3-4 µm
Excipulum ectal et medulla en textura globulosa-angularis, constitué de cellules globuleuses à subglobuleuses, à paroi légèrement épaissie, brun olivâtre foncé en NaCl iso., jusqu'à 15 µm de diam.
Merci de nous aider, amitiés à vous tous !
Roland
M. ligni I know only from wood. And it is characterized by inamyloid asci (though I have one collection from England on Larix with amyloid asci, which is probably something else.
I assume you have a Mollisia, not a Pyrenopeziza. But without living paraphyss this is difficult to be sure.
Cpuld you please send me the plate in higher resolution? You figure the spores as almost eguttulate but th two asci on th upper right contain spores with large guttules I think. Did you test these also with iodine? They look like a Helicogonium (intrahymenial parasite). If this is true they should be inamyloid.
Zotto
(zotto@arcor.de)
I am quite sure that the asci with big oil drops are a parasite (Helicogonium trabinelloides probably). You can easily test this by adding iodine to such asci - they must be inamyloid.
It is a pity that the collector has no microscope, as I understood, and cannot have a look at the fresh sample. So I cannot exclude a Pyrenopeziza here, especially since the substrate is herbaceous. The Helicogonium makes a Pyrenopeziza even more probable, as I use to see it as a parasite in this genus.
Perhaps the first record for N-America of this Helicogonium.
Zotto
Excuse me but I don't understand very well your response.
The big oils are in the ascospores not in the asci.
The iode have been added to asci and give an amyloid reaction.
You can see well this on the upper wright in the plate.
I don't understand when you whrite about the parasite Helicogonium trabinelloides.
It may be this disco or a parasite of this disco ?
Please, would you explain better for me.
If I have a work or a search to go further, I would like to be able to do so.
Thank you Hans for your support !
Merci, Roland
This parasite may occur inn all apothecia of your collection, or only in some of them. If you are unlucky, you will not detect it again. But f you detect it once more, then please test Lugol for the apical ring, it should be negative.
If you wish to read I can send you my paper on Helicogonium. But it is 11.5 MB
Zotto
Il a été hardu de photographier ces asques.
What do you think of this Hans !
Roland
Zotto
I would like to receive your document on Helicogonium.
Amitiés ! Roland
Je l'ai lu et il est passionnant.
More, I have change the texte on the photo of ascus just above.
Amitiés de Québec !
Roland, Jacqueline & Jules
Merci, Roland
Merci, Roland
Zotto