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Lophiostomataceae again...on Syringa
Björn Wergen,
27-02-2011 00:35
Hilast week i have found the next Lophiostomataceae, this time on a hanging, but dead stem of Syringa. Just look at my photos ;)
Frutbody is a pseudoperithecia 0,1 mm in diameter, with a short beaked ostiolus, black, after cutting it shows a whitish inside.
Spores mostly 20-24x5-6 µm, very rarely over 30µm, with a central septation and 2 vacuoles in each cell, hyaline, smooth, constricted at septa and swollen on both sides of the septa.
It would be great if somebody could helpme with this, unfortunately Ellis&Ellis (1997) does not mention any fungi on Syringa except a Microshaera ;)
regards and thanks,
kazuya
Lophiostoma? Macro (x 40)
Björn Wergen,
27-02-2011 00:36
Re:Lophiostomataceae again...on Syringa
micro
Alain GARDIENNET,
27-02-2011 08:30
Re:Lophiostomataceae again...on Syringa
Perhaps a species next to Lophiotrema vagabundum, but you should see a sheath around ascospores. Have you look at them in ink ?
Alain
Alain
Björn Wergen,
27-02-2011 12:27
Re:Lophiostomataceae again...on Syringa
ink = lactophenol? I can do it because I have the finding here. In water and with oil immersion there was no sheath, or it was not visible. I will make some more photos from it, perhaps it helps ;)
Alain GARDIENNET,
27-02-2011 19:29
Re:Lophiostomataceae again...on Syringa
Hi Kazama,
You can try with Indian ink or ink for foutain pen.
In water we sometimes can see sheath on young ascospores.
Good luck,
Alain
You can try with Indian ink or ink for foutain pen.
In water we sometimes can see sheath on young ascospores.
Good luck,
Alain
Gernot Friebes,
27-02-2011 19:34
Re:Lophiostomataceae again...on Syringa
Use Indian Ink (Tusche). Put a drop next to the coverslip and let it infiltrate until it reaches the spores. Then you should see a hyaline sheath, if it is present.
Best wishes,
Gernot
Best wishes,
Gernot
Björn Wergen,
27-02-2011 22:55
Re:Lophiostomataceae again...on Syringa
hi again
I saw this sheath around young spores, and sometimes around some older spores too ;)
I saw this sheath around young spores, and sometimes around some older spores too ;)