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Ascomata shaped like deformed black grains, measur
Bit at a loss: Cyathicula?
Maria Plekkenpol,
06-08-2023 10:26
On a small twig of probably Betula there were these cups with short thick stem. Fb small, 1,3 mm, stem 0,4 mm. Brown with ochre hymenium, outside looking fibrillose/hairy. Asci cylindrical, 90-115 x 7-10,5, 8 spored (biserial). Croziers +, IKI +. Cristals (?) at base of quite a few asci. Paraphyses slightly longer than asci, slender without capitate apex. Textura porrecta (I think?), endhyphae brown and very incrustated. Spores allantoid, almost bacilliform: 12,3-14,4-16,6 x 3-3,3-3,6.
Q= 3,7-4,4-5. Very little content within spores, no clear/bigger guttules.Â
I can't find a match within either Rutstroemia nor Cyathicula (which in the Netherlands they call Crocicreas). Does any of you have an idea? Thanks already!Â
Maria
Hans-Otto Baral,
06-08-2023 12:01

Re : Bit at a loss: Cyathicula?
Interesting! This is a member of the Rutstroemia tiliacea group. There is little consense about the species concept and host specificity. Available sequences from different substrates are very similar to each other.
I have a folder which also includes a subfolder Betula, a collection by M.A. Ribes.
Where did you find this? Is it collected from yesterday?
Maria Plekkenpol,
06-08-2023 12:30
Re : Bit at a loss: Cyathicula?
Thanks for the reply Zotto. My friend found it when we were in an area on clay, at the bank of a sand quarry. Trees there were Betula, Salix and Crataegus. That was last Friday.
https://www.google.com/maps/@51.8679207,6.3353405,222m/data=!3m1!1e3?entry=ttu
If you copy the link you can see it. It is the part left of the little building (left on the image). I can't open the folder alas. Can you tell a little bit more about this or do you have another paper?
Greetings, Maria
https://www.google.com/maps/@51.8679207,6.3353405,222m/data=!3m1!1e3?entry=ttu
If you copy the link you can see it. It is the part left of the little building (left on the image). I can't open the folder alas. Can you tell a little bit more about this or do you have another paper?
Greetings, Maria
Hans-Otto Baral,
06-08-2023 17:25

Re : Bit at a loss: Cyathicula?
My link does not work?
You can try starting here:
Go Ascomycetes illustrations, copy link and go to Ascomycetes.
Helotiales. Not sure if the sorting is after the alphabet.
There is an article about R. punicae. It is Peric & Baral, Mycol. Monten. XX, 2017 (2019): 167-189
TWO SPECIES OF THE GENUS RUTSTROEMIA
(RUTSTROEMIACEAE, HELOTIALES) NEW FOR MONTENEGRO:
R. FRUTICETI AND R. PUNICAE SP. NOV
(RUTSTROEMIACEAE, HELOTIALES) NEW FOR MONTENEGRO:
R. FRUTICETI AND R. PUNICAE SP. NOV
I can send you by mail
Maria Plekkenpol,
06-08-2023 20:08
Re : Bit at a loss: Cyathicula?
Thanks!! I downloaded and read the article, very interesting!!
As you know I am really an amateur, but from what I read R. punicae comes closest to my find.Â
So forgive me the following questions:
- Is it possible to already assume that it is R. punicae?
- Would you like me to send material?
If you want me to send material, please mail me the adress on m.plekkenpol@emhg.nl. Then I will send it tomorrow.
Thanks for your time and greetings,
Maria
As you know I am really an amateur, but from what I read R. punicae comes closest to my find.Â
So forgive me the following questions:
- Is it possible to already assume that it is R. punicae?
- Would you like me to send material?
If you want me to send material, please mail me the adress on m.plekkenpol@emhg.nl. Then I will send it tomorrow.
Thanks for your time and greetings,
Maria
Hans-Otto Baral,
06-08-2023 21:33

Re : Bit at a loss: Cyathicula?
I am sorry I have no time for study. But my suggestion is to send a portion for sequencing the ITS. If you do not know anyone, I suggest Kadri Pärtel from Estonia, she might be interested (I can ask her).
Maria Plekkenpol,
07-08-2023 07:33
Re : Bit at a loss: Cyathicula?
Please do and if she is interested I will send it to her.
She can email me her adress then. Thanks for all the help again!
Greetings, Maria
She can email me her adress then. Thanks for all the help again!
Greetings, Maria