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15-12-2025 07:09

Danny Newman Danny Newman

indet. Rutstroemiaceae sp. on unk. fallen leavesMc

15-12-2025 21:47

Pol Debaenst

Good evening, On 12/11/2025 I found ascomycetes w

15-12-2025 21:11

Hardware Tony Hardware Tony

Small clavate hairs, negative croziers and IKI bb

15-12-2025 15:54

Johan Boonefaes Johan Boonefaes

Unknown anamorph found on the ground in coastal sa

15-12-2025 15:48

Danny Newman Danny Newman

Melanospora cf. lagenaria on old, rotting, fallen

15-12-2025 07:05

Danny Newman Danny Newman

Pseudosclerococcum golindoi (det: Zotto)near Cosb

15-12-2025 11:49

Danny Newman Danny Newman

ITS sequences from the following two collections B

15-12-2025 12:34

Danny Newman Danny Newman

indet. Rhytismataceae on oak leafnear Purchase Roa

09-12-2025 12:06

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

13-12-2025 17:26

Buckwheat Pete

Hello everyone,I have a rather interesting ascomyc

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Monilinia johnsonii
B Shelbourne, 03-04-2024 20:09
B ShelbourneIt seems that this species is easily determined from the teleomorph by the habitat and macro, but I did some microscopy anyway.

Habitat: On overwintered, stromatised haws (fruit) of Crataegus monogyna, in litter or buried, in a mature glade, on a small hill, South Downs, England, late March with the first leaves of the host.

Associates: Lots of nematodes in the soil around the haws, one basidioma of Mycena acicula on a small twig, often with some herbaceous ground cover.


Apothecia: Measurements including full stipe length (3) 7-20 (30+) x 4-7 mm, n = 9, mean = 13 x 5 mm, Q = (0.8) 1.5-3.9 (4.2), mean Q = 2.3, goblet-shape, stipitate, cyathiform to cupulate and eventually +/- plane, deep to light brown, apparently darker with age, blackening when over mature, generally well-camouflaged amongst old upturned half-pits; receptacle and stipe with downy appearance; margin distinct, frosted-pruinose appearance, often in-rolled, with fringe of encrusted hairs/glassy processes?; disc concave, smooth appearance, more creamy colour; stipe 2-27+ x 1-1.5 mm, length depending on the depth that the haw is buried, when longer usually undulating more, appearance like the receptacle, often blackening near the base.


Storage and methods: Stored in a damp box for ~36 hours while still attached to haw, then stipe removed, cut in half, and slices taken from each side of the inside edge (not very effective as need more practise), mounted in water, some squashing later to separate the cells of the hymenium, IKI added to water mount, lots of bacteria in water mount.


Asci: Cylindrical, apex rounded to obtuse, croziers, IKI strongly bb – sclerotinia type, 1-1.5 seriate, one spore at apex, no inversely oriented spores observed.
- Living : ~130-190 x 9-12 um, pars sporifera ~20%, sub-biseriate when fully turgid, spores obliquely arranged, little to no apical thickening visible.
- Dead: ~7.5-9 um wide, pars sporifera > ~60%, uniseriate, apex more obtuse, distinct apical thickening when dead ~2.5-3 (4.8) um high.


Spores: Ellipsoid-obovate, often heteropolar with the apex rounded and the base more acute, occasionally more limoniform, rarely slightly curved, appearing binucleate with numerous medium-size shadowy LBs towards each pole and surrounding the nuclei, OCI 2-3, thick-walled, appear to have a perispore.
Measurements of mature vital spores in water mount: (11.4) 12.3-13.8 (14.1) × (6.3) 6.4-7.5 (7.6) µm, n = 21, mean = 12.9 × 7 µm, Q = (1.6) 1.7 - 2 (2.1), mean Q = 1.8.


Exudate: Orange-red, in subhymenium and medullary excip., appears more patchy in ectal excip., some clumping on surface; rhomboid crystals of varying sizes, hyaline, often in groups, apparently on the ectal excip.; appears to be a hyaline exudate or glassy processes around the marginal hairs.


Paraphyses: Cylindrical, apices rounded, slightly inflated to slight tapering - 3.5-4 (5) um wide, multi-septate, appear to have large hyaline guttules, possibly sensitive to trauma.


Ectal excipulum: Thin layer of hyphae externally with short hyphal tips/hairs protruding, then textura prismatica-globosa.


Marginal hairs: Like paraphyses, apical cell more inflated and capitate, sometimes irregularly inflated, protruding above the hymenium.


Subhymenium and medullary excipulum: Difficult to distinguish, textura intricata, separated from ectal excip. by broad band of horizontal hyphae.

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