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Thomas Læssøehttps://svampe.databasen.org/observations/10527903
Tympanis on Pinus
Juuso Äikäs,
26-03-2024 17:14
The primary spores appear to be first clavate inside the asci, then develop several septa, and finally split into thousands of secondary spores.
Could this perhaps be Tympanis confusa, or is it even possible to tell the species?
Josep Torres,
27-03-2024 09:42
Re : Tympanis on Pinus
Hola Juuso.
Algo muy parecido a lo que nos muestras y sobre el mismo sustrato lo mandé a secuenciar y el resultado fue que se trataba de Tympanis pini NR_173027
https://www.fungipedia.org/setas-informacion-y-consultas/19-microscopia/73530-un-perfecto-desconocido-tympanis-pini-crous-r-k-schumach.html#106494
Saludos.
Algo muy parecido a lo que nos muestras y sobre el mismo sustrato lo mandé a secuenciar y el resultado fue que se trataba de Tympanis pini NR_173027
https://www.fungipedia.org/setas-informacion-y-consultas/19-microscopia/73530-un-perfecto-desconocido-tympanis-pini-crous-r-k-schumach.html#106494
Saludos.
Juuso Äikäs,
27-03-2024 17:15
Re : Tympanis on Pinus
Thanks for the suggestion Josep.
My finding didn't have those large globose spores, so I think this might be something different.
I found the paper Reassessment of Tympanis based on types of ascospore germination
within asci, and Tympanis confusa is the only species that fits from the ones included in it.
I did a few measurements and got 27.7 - 31.4 × 4.1 - 5.2 µm for the clavate/septate spores, 2.9 - 3.3 × 1.0 - 1.2 µm for the tiny ones and 93.4 - 96.1 × 16.5 - 22.4 µm for the asci.
The spores and asci are a bit wider than in the paper, but otherwise everything would seem to fit T. confusa. The study is half a century old though, so I don't know how relevant it is these days...
My finding didn't have those large globose spores, so I think this might be something different.
I found the paper Reassessment of Tympanis based on types of ascospore germination
within asci, and Tympanis confusa is the only species that fits from the ones included in it.
I did a few measurements and got 27.7 - 31.4 × 4.1 - 5.2 µm for the clavate/septate spores, 2.9 - 3.3 × 1.0 - 1.2 µm for the tiny ones and 93.4 - 96.1 × 16.5 - 22.4 µm for the asci.
The spores and asci are a bit wider than in the paper, but otherwise everything would seem to fit T. confusa. The study is half a century old though, so I don't know how relevant it is these days...
Adam Polhorský,
27-03-2024 19:02
Re : Tympanis on Pinus
Hi Juuso, T. confusa is probably the only Tympanis growing on wood. Look here for more recent info https://doi.org/10.11646/phytotaxa.411.3.2
Juuso Äikäs,
28-03-2024 17:45
Re : Tympanis on Pinus
Thank you for the comment Adam. The only Tympanis growing on wood sounds interesting, but unfortunately I don't have access to the article.
Adam Polhorský,
28-03-2024 18:59
Re : Tympanis on Pinus
Sci-Hub should have the article
Lothar Krieglsteiner,
29-03-2024 09:31

Re : Tympanis on Pinus
Hello together,
fijst: I would also like ito receive the file!
Second:
T. confusa the only Tympanis species on wood? Do you mean on decorticated wood, without any bark on it? Then I can agree. Otherwiese Tympanis conspersa on Malus, Sorbus etc. is one and not the only example for Tympanis species growing on woody substrates.
Yours, Lothar
fijst: I would also like ito receive the file!
Second:
T. confusa the only Tympanis species on wood? Do you mean on decorticated wood, without any bark on it? Then I can agree. Otherwiese Tympanis conspersa on Malus, Sorbus etc. is one and not the only example for Tympanis species growing on woody substrates.
Yours, Lothar
Adam Polhorský,
29-03-2024 17:30
Re : Tympanis on Pinus
Lothar, yes I should've been more clear. On decorticated xeric wood. This species seems to be consistent in it. Other species grow on corticated branches.
Please try sci-hub, if it doesnt work, Ill send it to both of you.
Lothar Krieglsteiner,
29-03-2024 17:40

Re : Tympanis on Pinus
thank you, Adam! -
And: I received the file from Juuso in the meantime.
And: I received the file from Juuso in the meantime.