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Hi everyone, I found this weird asco growing on wo
Calycellina?
Ethan Crenson,
10-11-2023 07:01
Hello,
Today in a Brooklyn, NY cemetery on an oak leaf I found a small cluster of minute white cups with hairs at the margin. My first thought was Hyaloscypha, but the microscopy seems to suggest otherwise. I am wondering if this is Calycellina.
Apothecia smaller than 1mm in diameter growing on Quercus leaf (I think).
Asci 4-spored, IKI+, clavate, 32-37 x 5.2-6.0µm
Spores fusiform, usually with one acute end and on rounded end, somewhat curved, 9.5-15.1 x 2.2-2.9µm
Paraphyses septate with oil content. Dextrinoid in Lugol's.
Excipulum textura globulosa / intricata. Cells are often trapezoidal.
Hairs? Not sure.
Am I on the right track with Calycellina?
Thanks,
Ethan
Hans-Otto Baral,
10-11-2023 09:38
Re : Calycellina?
Yes, this is Calycellina lachnobrachya. One of my old papers deals with 4-spored Calycellinas. Regretabbly, both this and araneocincta are so far unsequenced, only ulmariae and flaveola are.
Ethan Crenson,
10-11-2023 14:05
Re : Calycellina?
Thank you again! I don't have much material left over, but I will try to sequence this collection if I can.
Was the paper you wrote on 4-spored Calycellinas is in Czech Mycology or Zeitschrift für Mykologie? I am trying to find it online.
A very interesting asco. I don't think I have seen an IKI reaction like that at the ends of the paraphyses.
Ethan Crenson,
10-11-2023 14:55
Re : Calycellina?
I think the reference I'm looking for is this:
Baral, H.O. (1989). Beiträge zur Taxonomie der Discomyceten II. Die Calycellina-Arten mit 4sporigen Asci. – Beitr. Kenntn. Pilze Mitteleuropas 5: 209–236.
I can't seem to find it online. Do you know where it is available?
Hans-Otto Baral,
10-11-2023 22:03
Re : Calycellina?
I sent it to you by mail.
Ethan Crenson,
20-11-2023 18:38
Re : Calycellina?
Thank you for the paper.
I have managed to get a fairly decent sequence from this collection. If you visit:
...there is an observational field for DNA Barcode ITS in the right column where I have added the results.