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Allophylaria nervicola - confirmation
Mario Filippa,
06-12-2022 23:07
I think I have found Allophylaria nervicola but I would like confirmation.
Several apothecia on dead leaves of Alnus, very wet. 20 november 2022, WWF Oasis "Il Verneto", Rocchetta Tanaro, Asti, Italy.
The apothecia are very small, not more than 0,5 mm, and scattered (impossible to photograph more than one at a time) on the leaf veins; white, then slightly reddish when old.
Asci and spores are somewhat similar to those of the common Calycellina alniella, but spores are almost without oil and asci are without croziers.
The spores are 8,5-13,5 x 3-3,5 µm in water. Living asci with spores inside 70-82 x 6,5-8 µm.
Paraphyses cylindrical, tips almost not enlarged, with refractive contents in elongated drops, often difficult to see (rather few in number, and maybe also disappearing or degenerating in the microscope mount?). However the refractive liquid remains into the mount around the apothecium, as seen in the photos.
Ascus pore hemiamyloid (red-brown in IKI).
Ectal excipulum a textura oblita, continuing from the hyphae of the stipe, hyphae that are cylindrical.
In the last photo is shown an old, reddening ascoma; it is mounted in NH4OH but that did not affect the colour.
Thanks for any advice
Mario Filippa
Hans-Otto Baral,
07-12-2022 09:36
Re : Allophylaria nervicola - confirmation
The asci should have croziers, from your photo it looks without but to be sure you need a photo of best young asci still attached to the hyphae. VBs in paraphyses were present?
The spores look very slighly heteropolar which makes me uncertain.
Living asci of A. nervicola are usually 100-130 x 9-10 µm large. Your asci could partly be alive but surely not fully turgescent, i.e. fully mature.
Mario Filippa,
07-12-2022 10:16
Re : Allophylaria nervicola - confirmation
Thank you for the answer, I'll try to take better photos of the ascus base and the paraphyses, as soon as possible.
What about the reddening of the old apothecia, is it normal?
What about the reddening of the old apothecia, is it normal?
Hans-Otto Baral,
07-12-2022 10:21
Re : Allophylaria nervicola - confirmation
I suppose that the VBs are responsible for such a color change. I overlooked you had described them. Perhaps you can see the colour inside dead paraphyses in a water mount?
Mario Filippa,
10-12-2022 23:22
Re : Allophylaria nervicola - confirmation
Well, I'm not satisfied at all of my new photos. The collection was made more than 20 days ago and I had to dry leaves and fungi because they can't resist anymore. The images I upload here are still from fresh specimens, but somewhat "tired" and I cannot see the VBs anymore, so no new photos of the paraphyses.
Images of asci are taken in methylene blue. I have seen the terminals of the ectal excipulum, not a great shot but they are there, in water.
However, the "tired" and not turgescent asci (measured in water) were 50-60 µm long, so shorter than expected. And still without croziers.
I will try in the next days to find some other specimens.
Thanks
Mario
Hans-Otto Baral,
11-12-2022 10:05
Re : Allophylaria nervicola - confirmation
This is all quite good and I now also accept the asci as simple septate, no doubt! The spores and asci are actually much smaller than in A. nervicola. I do not find a comparable collection in my files. So I created a new folder.
The liquid around the asci is artificial I think. I frequently met such efffect but could not explain, perhaps contmaination during preparation.