19-04-2026 21:23
Steve ClementsBonjour, I found this anamorphic fungus on old pl
19-04-2026 20:46
Steve Clements1 mm diameter approx spherical conidiophores on pl
12-04-2026 17:56
Hardware Tony
Found on dead stems in February earlier this year
17-04-2026 19:16
Enrique Rubio
Hi to everybodyI would appreciate any assistance r
14-04-2026 05:32
Ethan CrensonHi all, A few weeks back a friend pointed out som
17-04-2026 15:14
Bruno Coué
Bonjour.Récoltes du 16/04/2026, sur feuilles mort
12-04-2026 15:52
Gernot FriebesHi,I'm looking for help with this anamorph collect
14-04-2026 21:52
Gernot FriebesHi,found on dead leaves of Carex elata. Conidia: 4
16-04-2026 22:09
Buckwheat PeteHello, I'd like to ask about this older specimen:
15-04-2026 19:33
Fátima Durán ManzanequeHi!! I need help, I found this Ascomycete but I d
Réaction à la paraphénylènediamine
Gonzalez Garcia Marta,
20-03-2022 10:23
J'étudie un Rhizocarpon et j'ai besoin de connaître sa réaction à la paraphénylènediamine pour l'identifier. C'est la première fois que j'utilise ce réactif et je ne sais pas si je dois considérer ma réaction comme positive ou négative. Je m'attendais à un contrecoup comme Xanthoria avec KOH, mais ce n'est pas le cas. De plus, les cristaux de paraphénylènediamine sont devenus roses mais le thalle est inchangé.
Au départ, j'avais mis les cristaux dans un verre et dilué l'éthanol dessus, mais étant tellement volatils, j'ai mis les cristaux directement sur le thalle du lichen en laissant tomber l'éthanol. Est-il possible que ma technique ne soit pas adéquate?
J'aimerais connaître l'avis de quelqu'un qui a étudié ce type de lichens.
Merci.
Franz Berger,
20-03-2022 22:03
Re : Réaction à la paraphénylènediamine
Best to consult lichenological books for the proper application of P for determination!
P in alcohol 96% is nor stabiel for more than 3 days, Your image seems to be the result of application of too old reagent?
So, it is recommended to keep all specimens back where this this reaction is necessary and make a new solution for these. Dark coloured sufaces dont show a distinct reaction, a cut to uncover the mark layer is favorablie some times. CAVE, alcoholic solutions are running very quick into the specimen, so try only at a part at the margin and omit the tinged part afterward.
Reactions:
no reaction = P-
possible reactions are P+ red (e.g. Fumarprotocetraacid; P+orange P+ yellow (prsoromic acid); it should be noticed, if the reaction is running slowly or quickly.
CAVE: This reagent causes irreversible aweful stains on surfaces like wood or also stones!
Hoping to help You with this
Franz
P in alcohol 96% is nor stabiel for more than 3 days, Your image seems to be the result of application of too old reagent?
So, it is recommended to keep all specimens back where this this reaction is necessary and make a new solution for these. Dark coloured sufaces dont show a distinct reaction, a cut to uncover the mark layer is favorablie some times. CAVE, alcoholic solutions are running very quick into the specimen, so try only at a part at the margin and omit the tinged part afterward.
Reactions:
no reaction = P-
possible reactions are P+ red (e.g. Fumarprotocetraacid; P+orange P+ yellow (prsoromic acid); it should be noticed, if the reaction is running slowly or quickly.
CAVE: This reagent causes irreversible aweful stains on surfaces like wood or also stones!
Hoping to help You with this
Franz
Gonzalez Garcia Marta,
21-03-2022 13:19
Re : Réaction à la paraphénylènediamine
Muchas gracias Franz.