Unknown find on Salix - Forum ASCOFrance

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Unknown find on Salix

Dirk Gerstner, 11-02-2022 09:00

Hello all,

I found this small yellow ascomycete on a rotten willow branch on 05.02.2022.
Unfortunately there was only one. That's why there are so few microfeatures.
The branch is damp, maybe there is still growth.

Size of the FK approx. 1- 1.2 mm.
Microfeatures see appendix.
Spores [95% - 8 - QPr - v - H2O(nat) ] = 6,5 - 8,4 - 10,4 x 2 - 2,7 - 3,3 µm; Q = 2,5 - 3,9.
OCI 1
Spore shape you can judge yourself, i dont know the correkt name for the form.
I think I saw spores with cilia in the preparation.
Porus reaction negative even with pre-treatment KOH.
Ascus base hook+

Could it be a Mollisia?

Kind regards and thank you very mutch
Dirk

Nicolas VAN VOOREN, 11-02-2022 09:12

Re : Unknown find on Salix

I think it is a Hyalorbilia

Hans-Otto Baral, 11-02-2022 09:24

Re : Unknown find on Salix

Yes, and I suppose H. subfusispora or perhaps H. fusispora.

Fusiform spores with globose spore bodies (disappearing in KOH).

The large apo diameter speaks more for H. fusispora, because H. subfusispora hardly exceeds 0.5 mm.

The excipulum is of hyaline prismatic cells, very different from Mollisia.

Dirk Gerstner, 11-02-2022 21:43

Re : Unknown find on Salix

Hello, you two,

Thank you for your quick answers.
The genus is completely new to me, but I have compared the characteristics as well as possible with your monograph. The waxy, hygrophane appearance of the fruit body also fits very well there, which is not so good to see in the photo.
To determine the species now exactly as Hyalorbilia_fusispora, I lack the experience.

But thank you again.
Kind regards
Dirk

Hans-Otto Baral, 12-02-2022 08:04

Re : Unknown find on Salix

The distinctiojn is complicated. One should see enough spores with their spore bodies (which are KOH-soluble unlike lipid bodies that are also present). The species concepts in this genus are often still only tentativem but it was clear that species with fusiform spores are not always the same.

So when your scale corresponds to 1 mm, it is quite clearly not H. subfusispora.

Dirk Gerstner, 12-02-2022 08:44

Re : Unknown find on Salix

Hello,
yes the scale is 1mm.
The spore illustrations in your "folder fusispora" exactly match the ones I have seen, which unfortunately I cannot check anymore is,is the reaction of the spores to KOH.
But it's good to know this if similar findings turn up again.

And so I think and hope that the find can be filed with H._fusispora cf.

Thank you for your support!

Lothar Krieglsteiner, 12-02-2022 09:41

Re : Unknown find on Salix

Hello Dirk,
you don`t need to check if the spore bodies are KOH soluble. They are (at all orbilioid species). They are dissolved when the spore dies.
Yours, Lothar

Hans-Otto Baral, 12-02-2022 10:23

Re : Unknown find on Salix

Yes, but in Hyalorbilia one cannot easily decide which globose bodie is an SB and which an LB. Then KOH helps, or Cresyl blue. But it is not easy: the KOH test is best done with spores inside asci, so that they do not fleat away, and CRB enters the spores only slowly.

Dirk Gerstner, 12-02-2022 20:06

Re : Unknown find on Salix

Hello Lothar, hello Hans-Otto,

thank you very much for your interesting answers.
As an absolute layman in this matter, one must understand this first. But on this way one can only learn.
Maybe I can find some more orbilioid species to try the whole thing out again.

Herzliche Grüße
Dirk

Lothar Krieglsteiner, 12-02-2022 20:11

Re : Unknown find on Salix

Hallo Dirk,
übrigens freue ich mich, hier von Dir zu lesen.
Aller Anfang ist schwer - und Orbilia sicher bersonders.
Herzliche Grüße von Lothar

Dirk Gerstner, 12-02-2022 20:39

Re : Unknown find on Salix

Hallo Lothar,

danke und gerne bin ich hier, vor allem bei solch netten Leuten.
Ja, ab und zu versuche ich auch Ascomyceten zu bestimmen. Meist scheitere ich ober brauche Hilfe von
Freunden unnd Gleichgesinnnten.
Aber der Anfang ist gemacht.

Liebe Grüße auch daheim
Dirk

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