13-04-2025 22:47
éric ROMERO
Bonjour, Je sollicite votre avis pour cette colle
13-04-2025 13:00
Zuzana Sochorová (Egertová)
Hello,I guess the best option how to label this co
13-04-2025 10:21
Castillo Joseba
Mer mandan el material de Galicia (España), rec
10-04-2025 16:22
Bernard CLESSE
Bonjour à toutes et tous,Pourriez-vous me confirm
12-04-2025 19:49
Andgelo Mombert
Bonjour,Un ascomycète qui me pose problème.Ascom
10-04-2025 10:09
Mathias Hass
Hi, Found in March on last years stick from Rubus
10-04-2025 21:52
Andreas Millinger
Bonsoir! I'm new to the forum and would kindly as
11-04-2025 15:00
Vasileios Kaounas
Found in rotten wood of Abies cephalonica or Popul
03-04-2025 21:31
Francois Guay
Hi, I found this interesting ascomycete on incuba
Cosmospora and its possible anamorph
Stephen Martin,
31-12-2021 11:34
Dear colleagues. First of all I wish everyone a better new 2022 and hope things get back to normal soon.I am studying a Necteriaceae, possibly a Cosmospora it was similar to this entry in the af db :
http://www.ascofrance.fr/search_recolte/2236
I have managed to isolate it as an anamorph but before further tests I want to confirm that the Anamorph I isolated do make sense. the mould produced those orange pustules which I saw in some site for cosmospora sp. so I think we are on the right track. The spores looks like that in the database labelled Cosmospora sp. by ALAIN GARDIENNET (link above). The hyphae have numerous oil bodies refractive bodies. I could not detect obvious conidiophores (maybe they just bud from simpile conidiophore-like branches. Spores 10-16 um long. It does not look like a fusarium :-(
The Perithecia were growing on some black Xylariaceae stomata attached on fallen olive branches. They were pyriform in my opinion.
- - - - bottom line - - - -
There was also some kind of contaminant fungus, so I subcultured on different media to try and have a pure culture. I would also be interested to know what is that 'contaminant fungus' too.
Thanks and hope this is interesting after lots of work! (I should take some more pics!)
There is a paper by Herera et al (2015) dealing with Pseudocosmospora....
Stephen Martin,
31-12-2021 11:36
Re : Cosmospora and its possible namorph
Contaminant
Christian Lechat,
31-12-2021 15:42
Re : Cosmospora and its possible anamorph
Hi Stephen,
Have you identified the host? This is very important characteristic in order to have a chance to know what your cosmospora-like fungus is.
Did you see asci and ascospores in your collection? It would be useful to make a vertical section of the ascomatal wall.
Have you identified the host? This is very important characteristic in order to have a chance to know what your cosmospora-like fungus is.
Did you see asci and ascospores in your collection? It would be useful to make a vertical section of the ascomatal wall.
In fact, your fungus has no relation to Alain Gardiennet's collection, which is Macroconia leptosphaeriae, and have a fusarium-like asexual morph.
According your images of ascomata, I think it is a species of Pseudocosmospora
Good lucke and Happy new year.
Christian
Stephen Martin,
01-01-2022 08:32
Re : Cosmospora and its possible anamorph
Hi Christian.
I have forgot to finish my sentence and I was saying There is a paper by Herrera et al (2013) dealing with Pseudocosmospora.... where the anamorphs have the same spores.
https://www.tandfonline.com/doi/abs/10.3852/12-395?journalCode=umyc20
My question was initially to be sure that I got the right anamorph isolated, maybe I progress to DNA seq. I know Cosmosporas should have Fusarium anamorphs and manyStylonectria have acremoniums (I soon post about another finding) but I did not get those in my cultures. Hence yes, it seems we aredealing with a Pseudocosmospora. I hope antibiotics in the agar culture media would not interfere with the seq.
Regards the perithecia, I will show you the microscopy later today. The problem is that I cannot slice by hand and razor a section of a perithecium about 0.3 mm wide. The most I can cut is in half, then it just slips under the razor or break up. If mycologists manage to do those sections 0.1mm or less sections by hand I bow my hat i awe! I can at most show lateral views of isolated peritechia.
About the host, I currently have this pic. I try to get some spore images
I have forgot to finish my sentence and I was saying There is a paper by Herrera et al (2013) dealing with Pseudocosmospora.... where the anamorphs have the same spores.
https://www.tandfonline.com/doi/abs/10.3852/12-395?journalCode=umyc20
My question was initially to be sure that I got the right anamorph isolated, maybe I progress to DNA seq. I know Cosmosporas should have Fusarium anamorphs and manyStylonectria have acremoniums (I soon post about another finding) but I did not get those in my cultures. Hence yes, it seems we aredealing with a Pseudocosmospora. I hope antibiotics in the agar culture media would not interfere with the seq.
Regards the perithecia, I will show you the microscopy later today. The problem is that I cannot slice by hand and razor a section of a perithecium about 0.3 mm wide. The most I can cut is in half, then it just slips under the razor or break up. If mycologists manage to do those sections 0.1mm or less sections by hand I bow my hat i awe! I can at most show lateral views of isolated peritechia.
About the host, I currently have this pic. I try to get some spore images
Christian Lechat,
01-01-2022 10:50
Re : Cosmospora and its possible anamorph
Ok Stephen, let's wait for more information.
Also, the genus Cosmospora str. ss. has an acremonium-like asexual morph, while Stylonectria, Macroconia, Fusicolla, Dialonectria have a fusarium-like asexual morph.
Christian
Stephen Martin,
01-01-2022 19:51
Re : Cosmospora and its possible anamorph
For now I have some data on on the perithecia, asci and ascospores.
The perithecia seems to be fragiferiform-subpyriform, with a truncate apex. Wall warted (pustulate), bright red darkening at the top, paler below. When detached from the substrate, they measure about 0.23-0.29 mm in length and 0.19-0.23 in width (widest part). I couldn't produce a thin section!
The ascii cylindrical, sides parallel (hence not swollen or fusiform) flexuose, with ascospores sitting at about 45 degrees stacked over each other in one series along most of the ascus length, apex truncate, J-ve, 75-95 um long by 6.5-8.5um wide
Ascospores
Navicular-fusiform, broadest at the middle, ends broadly rounded, one septate and slightly constricted at the septum, measuring (12.8) 13.8 - 17 (17.5) × (4.7) 5.6 - 6.9 (7.6) µm // Mean = 15.2 × 6.3 µm ; Qe = 2.5 ; Ve = 320 µm3
Stephen Martin,
01-01-2022 22:06
Re : Cosmospora and its possible anamorph
Christian, to my astonishment I managed to cut fine sections of the perithecia (very nice in my opinion!) and also some images of the underlying fungus (not a Xylariaceae). I also have spores of the host. There is lot to digest but I think we have lot of data now.
Here I attach images of cross sections of the ascomata
Here I attach images of cross sections of the ascomata
Stephen Martin,
01-01-2022 22:13
Re : Cosmospora and its possible anamorph
Here are some images of the host under the stereomicroscope and its elongated spores (= Diatrypaceae ?)
Christian Lechat,
03-01-2022 11:53
Re : Cosmospora and its possible anamorph
I have to admit that I don't understand it anymore. There is a solution: you try the culture again to see if it gives the same thing as the first time, and if so you sequence it to find out where it is. Moreover, it is essential to know the identity of the host, without that it is almost impossible.
All the best,
Christian
Stephen Martin,
03-01-2022 21:11
Re : Cosmospora and its possible anamorph
Dear Christian,
Thanks... your reply means that this is very strange! Some quick questions maybe you can put some light on this foggy picture:
1. Why have we abandoned Pseudcosmospora sp. ? Are the ascospores too large ?
2. Then, can it be a Cosmospora sp. or either that match with the genus concept?
3. Can you provide further help on the host (genus level)? Are those elongated spores typical of some family or genus?
I would love to learn what is unpeculiar with the data I presented.
I try to re-isolate, but the secimen is getting contaminated from airborne spores which makes life quie difficult
Thanks... your reply means that this is very strange! Some quick questions maybe you can put some light on this foggy picture:
1. Why have we abandoned Pseudcosmospora sp. ? Are the ascospores too large ?
2. Then, can it be a Cosmospora sp. or either that match with the genus concept?
3. Can you provide further help on the host (genus level)? Are those elongated spores typical of some family or genus?
I would love to learn what is unpeculiar with the data I presented.
I try to re-isolate, but the secimen is getting contaminated from airborne spores which makes life quie difficult
Christian Lechat,
04-01-2022 04:04
Re : Cosmospora and its possible anamorph
Hello Stephen,
Cosmospora is characterised by an acremonium-like asexual morph in culture and green colony.
Pseudocosmospora also has an acremonium-like asexual morph, but rosy-buff, pale-luteous, or salmon-pink colony.
Cosmospora is characterised by an acremonium-like asexual morph in culture and green colony.
Pseudocosmospora also has an acremonium-like asexual morph, but rosy-buff, pale-luteous, or salmon-pink colony.
Cultures must be made in sterile environment, in laminar flow cabinet.
Good lucke
Christian
Stephen Martin,
04-01-2022 08:03
Re : Cosmospora and its possible anamorph
Acremonium-like anamorph and pale salmon colonies observed, but one colony had a a greenish hue when old. In one of the pics I think I got a pure colony (pinkish hue) and should make it a Pseudocosmospora but, yes not easy to draw a conclusion.
Ok, i think we have an interesting species and will progress with sequencing.
Many thanks...
