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Pyrenopeziza lignicolous IKI-
Viktorie Halasu, 24-12-2021 12:02
Viktorie HalasuHello,

wish you a merry christmas! 

In case someone drops by, I have this small Pyrenopeziza(?), on a piece of bark stuck 1m above ground between branches, on its inner side. The tree is Crataegus, I'm not sure about the piece of bark.
Asci seem inamyloid, mostly inflated with a narrow base.
Paraphyses cylindrical, VB-, not reacting in Baral's either.
Only short brownish clavate cells on the margin.
Soc. Orbilia xanthoguttulata. A row of trees between railroad and a field, riparian forest nearby.

Am I in a correct genus? I've seen just a few lignicolous species in Bernard's key and neither was a good match. Maybe it froze a bit overnight, perhaps a damaged Mollisia would be a possibility too?
Thank you in advance.
Viktorie
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Enrique Rubio, 24-12-2021 12:17
Enrique Rubio
Re : Pyrenopeziza lignicolous IKI-
Hi Viktorie
Close to Pyrenopeziza caespiticia?
Viktorie Halasu, 24-12-2021 12:27
Viktorie Halasu
Re : Pyrenopeziza lignicolous IKI-
Hello Enrique, 

that was one of the names I checked, but I haven't seen such long marginal cells in my fungus and I don't know if the paraphyses and marginal cells can loose VBs entirely when the rest of hymenium is alive. 
But this Piotr's collection seems to be close: http://ascofrance.com/search_forum/3296

Viktorie
Hans-Otto Baral, 24-12-2021 12:39
Hans-Otto Baral
Re : Pyrenopeziza lignicolous IKI-
I have also no other suggestion. The species is a bit difficult and can easily be misidentified. Nick Aplin wanted in a manuscript to describe a British collection as a new species but I considered it to be P. caespiticia. His unpublished sequence and another by Ingo Wagner/Florian Prell are quite close to each other. But Guy's sequence KY965813 is very distant and I tend to believe that it is Calycina vulgaris.
Enrique Rubio, 24-12-2021 12:49
Enrique Rubio
Re : Pyrenopeziza lignicolous IKI-
This fungus appears to be xero-tolerant. In my region this fungus is particularly abundant on Malus branches still attached to the tree. But I never see it on the ones that have fallen to the ground, unless they have fallen after a windstorm.
Michel Hairaud, 24-12-2021 15:07
Michel Hairaud
Re : Pyrenopeziza lignicolous IKI-
Bonjour les amis, 

I Used to call this species Mollisia caespiticia , particularly because of the mollisioid paraphyses contents (long VBs staining in CRB) 
Is it based on molecular analysis that you now consider it as a Pyrenopeziza ? 

Amitiés
Michel
Hans-Otto Baral, 24-12-2021 15:42
Hans-Otto Baral
Re : Pyrenopeziza lignicolous IKI-
There occur low-refractive VBs indeed.

The two sequences of M. caespiticia cluster supported with Heterosphaeria patella! That is no Mollisiaceae but akin to Pyrenopeziza. Several Pyrenopeziza spp. cluster more distantly and with less support with these.

Earlier I thought that M. caespiticia is related to M. ligni but it isn't.
Michel Hairaud, 24-12-2021 17:47
Michel Hairaud
Re : Pyrenopeziza lignicolous IKI-
Merci Zotto, 
Is there a paper I missed in which it has already been published ? 
IF still keeps Mollisia as current generic name
Michel
Nick Aplin, 25-12-2021 01:49
Re : Pyrenopeziza lignicolous IKI-
Season's greetings everybody,

I didn't publish anything, although I finally came to the conclusion my collections were better refered to as 'Mollisia' caespiticia rather than anything new. Much of the collection data is available in Zotto's Google Drive folders:
Helotiales -> Ploettnerulaceae -> Pyrenopeziza on wood and bark -> caespiticia 4.5-8.5 x 1.5-2 IKI- -> Helotiales 28-IV-2020

As Zotto suggests, the ITS sequence seemed to belong in Heterosphaeraceae rather than anywhere near Mollisia or Pyrenopeziza:

>Helotiales_sp_LGW_28.IV.2020_Salix_Branch
TAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGAGTTTTTGCCCTAACGGGTAGATCTCCCACCCTTGAATACATACCTTTGTTGCTTTGGCGGGCCGCGCTCGCGCTACTGGCTTGTCTAGTACGTGCCCGCCAGAGGACCACAACTCTTGTTTTTAGTGATGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTTCGAGCGTCATTATACCACTCAAGCTCAGCTTGGTATTGGGGCCCGCCCACCAGCGGCCCCTAAAGTCAGTGGCGGTGCCGGTCGGCTCTAAGCGTAGTAATACTCCTCGCTATAGGGTCCGGTCGGTTGCTTGCCAACAACCCCAAATTTTTATCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA

This collection seems identical to mine except for the slightly wider spores.

Amités,
Nick
Hans-Otto Baral, 25-12-2021 08:33
Hans-Otto Baral
Re : Pyrenopeziza lignicolous IKI-
Thanks Nick! In order to obtain a better insight, it is generally useful to compile the available data of samples and to see how far the variation goes. But it is alwaya dangerous when doing a joint description to include finds of different species. So far I did not try.