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02-11-2014 07:33

Beñat Jeannerot Beñat Jeannerot

Adio to all,Sorry to use again this method to foun

02-11-2014 16:04

Beñat Jeannerot Beñat Jeannerot

Adio to all,A question to Germans.I've seen a sign

01-11-2014 22:20

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Good evening, can this be something different than

27-10-2014 20:09

Peter Thompson

Hello Everyone,I have found a Gibberella species w

31-10-2014 14:59

Bernard Declercq Bernard Declercq

Hi there,Collected on water-soaked rotten wood of

31-10-2014 14:16

Garcia Susana

Hi:With this I do not know correctly interpret wha

31-10-2014 10:33

Gilbert MOYNE

Bonjour;Encore besoin de faire appel à votre bon

07-10-2014 17:19

Malcolm  Greaves Malcolm Greaves

Can someone help with this Scutellinia. Found a gr

30-10-2014 16:34

Alex Akulov Alex Akulov

Fungicolous hyphomycete on Lasiosphaeria strigosa

30-10-2014 16:39

Alex Akulov Alex Akulov

Nice synnamatous fungus from the unusual substrat

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Sub-millmeter whitish apothecia on subalpine humus
Edvin Johannesen, 02-09-2021 13:49
Edvin Johannesen
These tiny apothecia (less than 1 mm) were found in a moist depression under Salix in a mountain region in Central Norway. The substrate is nearly-decomposed plant material.  The macro photos are of fresh specimen. Unfortunately, it took som time (in the fridge) before I was able to do microscopy, so deliquescence has started. I was however able to find mature asci and spores (photos in water). Spores measure typically 11x4 microns. Multiple guttules (or spore bodies?) are seen towards the poles. Asci IKI- as far as I can see. Is it possible to ID (at least to genus) from this limited information?

Thanks.
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Mirek Gryc, 02-09-2021 16:08
Re : Sub-millmeter whitish apothecia on subalpine humus
Hi Edvin

Pezoloma?

Mirek
Edvin Johannesen, 02-09-2021 20:23
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
That's an interesting proposal, Mirek. A genus I have not encountered before. I will check further. Sad to say, the specimen is now in a poor condition.  Thanks!
Hans-Otto Baral, 02-09-2021 22:02
Hans-Otto Baral
Re : Sub-millmeter whitish apothecia on subalpine humus
LBs are for sure in the spores (you can test it with KOH, they resist). But how are the paraphyses? are they still alive and do they contain VBs?
Edvin Johannesen, 03-09-2021 14:34
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
Thanks, Zotto.  I was happy to find one apo apparently in good shape, and have studied it in water, KOH and Lugol. Some additional/corrected information.

1. The spore LBs resist KOH

2. Paraphyses contain VBs. These disappear in KOH and the paraphyses also become thinner in KOH.

3. Asci vary in being uni- or biseriate. Also the number of spores seem to vary, the normal being 8 (uni- or bi). In some asci the spores spread througout the asci, in other they tend to aggregate towards the apex.

4. Ascus apparatus is hemiamyloid (brownish in Lugol).

New photos are all in water (except the last one, in Lugol). I had to use phase contrast to get a clear image of the paraphyse VBs.

Does this help?
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Hans-Otto Baral, 03-09-2021 22:10
Hans-Otto Baral
Re : Sub-millmeter whitish apothecia on subalpine humus
Interesting! Could be a Pezoloma indeed. But I see no VBs. Your phase photo seems to refer to dead paraphyses. In water under bright field there are no apparent contents.
Edvin Johannesen, 03-09-2021 23:43
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
OK, I learn every day :)
Mirek Gryc, 04-09-2021 07:02
Re : Sub-millmeter whitish apothecia on subalpine humus
Hi
In fact ascomata already heavily damaged but I think the pictures show the contents paraphyses.
I allowed Edvin's photo correction.
Mirek
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