Hi Bo,

I do not know what Lachnum callunae is . Is it in the sense of Dascysyphus callunae Rehm ou a lately described species I overlooked ?

Your macro pictures shows a fungus with the appearence of a species common on Calluna (and other substrates) : L. virgineum , which is characterized by present croziers and no Vbs in paraphyses. Paraphyses are  protruding the asci by 20-30 µM and rather broad (5-7 µm) . All these characters you can check in water mounts.

Are you sure about the 3-3,25 µm broad ? From the scales you show, they appear about the distance between 2 strikes.

Amitiés

Michel

 

Hi Michel and thanks for your reply. Lachnum callunae Baral ined. is on the Danish list, but I don't know more.
I am new to microscope, but the calibration should be ok. The spores looks different in size, and surely I can be wrong on this matter. The distance between the strikes should be 2,25um, so some of the spores are rightly this size. Others looks a bit thicker on a new picture uploaded, but again pictures are taker with a Mobile phone, and perhaps not good enough. L. virgineum Is common in Denmark, so it seems more likely to be the one.
Thanks again.
Bo

Salut Michel et merci pour votre réponse. Lachnum callunae Baral ined. est sur la liste danoise, mais je n'en sais pas plus.
Je suis nouveau dans le microscope, mais l'étalonnage devrait être correct. Les spores ont une taille différente et je peux sûrement me tromper à ce sujet. La distance entre les frappes devrait être de 2,25 um, donc certaines des spores ont à juste titre cette taille. D'autres semblent un peu plus épais sur une nouvelle image téléchargée, mais encore une fois, les images sont prises avec un téléphone portable, et peut-être pas assez bonnes. L. virgineum Est commun au Danemark, il semble donc plus probable que ce soit celui-là.
Merci encore.
Bo

Hi Bo,

There actually is a Dasyscyphella callunea  (nom. prov.) in a Lachnum key by Zotto . It seems that hairs in your collection are verrucose till the top , so it matches with Lachnum

Michel

Thanks again Michel :)
I will look into the key you mailed.
Bo

Without docu of living paraphyses and ascus base & iodine reaction, as well as hair length I would not dare saying anything. Substrate alone is insuffucient for ID.

Thanks Hans-Otto
I will see if it's possible for me to document any of your suggestions on Monday. I don't have iodine, but perhaps some Melzer can be used instead.
Bo

Melzer is also iodine but not the best for discos. In this case it would be o.k., I do not know a hemiamyloid reaction in Lachnum on wood.

Thanks Hans-Otto
You are right I did not use immersion oil.
It ended up as Lachnum sp., but i did find a L. Calluna in 2023, which is accepted in Denmarks Svampeforum. It no easy.at all...:)

Can you give me the link to the post in that Svampeforum?

I tried to go to Svampeforum but cannot find any Lachnum

Here is link to my L. calluna at Svampeforum:

https://svampe.databasen.org/observations/10340943

Thanks, but are there any microscopic data?

Not mentioned can I see, and i cannot Remember if any sadly.
The 12 accepted danish finds are listed here. 
https://svampe.databasen.org/taxon/69628

Too bad, I was not aware of this. Seems impossible to detect such entries by web search.

Yes it is not easy to find these on the web. Confirm founds will also t hough figure on international web also.