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06-11-2024 20:07

B Shelbourne B Shelbourne

• Macro and habitat suggest Hymenoscyphus s.l.,

04-09-2024 21:02

Stephen Mifsud Stephen Mifsud

I have found an interesting Xylaria growing on fal

06-11-2024 11:59

Stephen Mifsud Stephen Mifsud

I am trying to identify a Cheilymenia sp. using ke

05-11-2024 18:00

Karen Poulsen

Hello, Can anyone help with this one? On twigs o

20-10-2024 16:48

Albert Meek

Globose sporesca. 16um, with  many drops. On bare

05-11-2024 10:35

Juuso Äikäs

These pale-yellow fruitbodies were growing on core

03-11-2024 17:36

B Shelbourne B Shelbourne

• Macro and habitat suggest Hymenoscyphus s.l.,

04-11-2024 22:58

B Shelbourne B Shelbourne

• Both Erysiphe and Phyllactinia species reporte

04-11-2024 20:28

Malcolm  Greaves Malcolm Greaves

A small group of Scutellinia found on a rotting lo

04-11-2024 17:32

Yves Antoinette

Bonjour, je pense qu'il peut s'agir de Trichoderma

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Is it Gliocladium ???
Stephen Mifsud, 23-10-2020 12:02
Stephen Mifsud

Hi, I found small white-cream colonies on decaying seeds of Washingtonia, which I cultured. The resulting colonies where fluffy ash-white with a widespread maube-violet central zone. Under the microscope, the first thing that was stricking was the irregular size of the spores, ranging from 5 to 10um long. Spore dimensions from pixmetere:
(5.7) 6.4 - 9.6 (10.4) × (3.3) 3.4 - 4.9 (5.9) µm
Q = (1.5) 1.6 - 2.2 (2.7) ; N = 27
Me = 8 × 4.2 µm ; Qe = 1.9


The spores were terminal, solitary on undifferentiated hyphae, similar to the mycelium, but sometimes they appeared to be in small clusters, and occassionally I saw a penicillate arrangement on very long conidiophore.


I dont know if this is some irregular or not well known Gliocladium sp.

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David Malloch, 23-10-2020 19:59
David Malloch
Re : Is it Gliocladium ???
Hello Stephen,

Perhaps this is a member of the Microascaceae. The habitat on decomposing seeds would be common for that family.  The conidiophores and hyphae remind me of Scedosporium or perhaps an early stage of a Graphium. Watch for ascomata of Petriella or Pseudallescheria in your cultures.

David
Stephen Mifsud, 23-10-2020 21:14
Stephen Mifsud
Re : Is it Gliocladium ???
Thank you David, I will continue my investigatoion in that direction you kindly suggested and I think you are correct. There are many things which are matching, habitat, habit, variety of conidiophores (short, long, synnematous), spores with truncate bases and if I understood the annelid conidiophores with reference to wrinkled sides (seen in one of my images without congo red). The cottony colony with a dark centre is also diagnostic.

This genus is rather complex forming different reproductive structures - myself I was confused if I have a contamination of two species.

I examined my cultures by taking a film of mycelia from the surface with cellophane tape. I will investigate further down. Pure subcultures are growing fine too. Seems to be a hard one re determination to species! 

Thank you for replying and sharing your knowledege!


Important references

http://website.nbm-mnb.ca/mycologywebpages/Moulds/Scedosporium.html
http://thunderhouse4-yuri.blogspot.com/2012/10/scedosporium-prolificans.html