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27-11-2020 11:34

DirkW DirkW

salut a tous,on dead stem of indet. herbaceous pla

27-11-2020 16:48

Andgelo Mombert Andgelo Mombert

Bonsoir, Je suis intéressé par de la documentat

26-11-2020 17:58

Gérard MELARD

Bonjour à chacune et à chacun,Je vous soumets un

26-11-2020 16:17

Blasco Rafael Blasco Rafael

Hola,  Alguno de Ustedes tiene documentación o a

27-11-2020 11:16

Joaquin Martin

Hi,I found this little ascomycete on rubus, I thin

24-11-2020 14:46

Thomas Læssøe

see: https://svampe.databasen.org/observations/101

11-02-2017 13:39

Nihad Omerovic

Hello,Loking for confirmation or opinions on this

26-11-2020 20:43

Marek Capoun Marek Capoun

Hello everybody, I need help with the determinatio

27-11-2020 07:51

Zuidland Peter

Can I get help with this small pyrenomycete please

27-11-2020 07:48

Zuidland Peter

Can I get help with this small pyrenomycete please

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Is it Gliocladium ???
Stephen Mifsud, 23-10-2020 12:02
Stephen Mifsud

Hi, I found small white-cream colonies on decaying seeds of Washingtonia, which I cultured. The resulting colonies where fluffy ash-white with a widespread maube-violet central zone. Under the microscope, the first thing that was stricking was the irregular size of the spores, ranging from 5 to 10um long. Spore dimensions from pixmetere:
(5.7) 6.4 - 9.6 (10.4) × (3.3) 3.4 - 4.9 (5.9) µm
Q = (1.5) 1.6 - 2.2 (2.7) ; N = 27
Me = 8 × 4.2 µm ; Qe = 1.9


The spores were terminal, solitary on undifferentiated hyphae, similar to the mycelium, but sometimes they appeared to be in small clusters, and occassionally I saw a penicillate arrangement on very long conidiophore.


I dont know if this is some irregular or not well known Gliocladium sp.

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David Malloch, 23-10-2020 19:59
David Malloch
Re : Is it Gliocladium ???
Hello Stephen,

Perhaps this is a member of the Microascaceae. The habitat on decomposing seeds would be common for that family.  The conidiophores and hyphae remind me of Scedosporium or perhaps an early stage of a Graphium. Watch for ascomata of Petriella or Pseudallescheria in your cultures.

David
Stephen Mifsud, 23-10-2020 21:14
Stephen Mifsud
Re : Is it Gliocladium ???
Thank you David, I will continue my investigatoion in that direction you kindly suggested and I think you are correct. There are many things which are matching, habitat, habit, variety of conidiophores (short, long, synnematous), spores with truncate bases and if I understood the annelid conidiophores with reference to wrinkled sides (seen in one of my images without congo red). The cottony colony with a dark centre is also diagnostic.

This genus is rather complex forming different reproductive structures - myself I was confused if I have a contamination of two species.

I examined my cultures by taking a film of mycelia from the surface with cellophane tape. I will investigate further down. Pure subcultures are growing fine too. Seems to be a hard one re determination to species! 

Thank you for replying and sharing your knowledege!


Important references

http://website.nbm-mnb.ca/mycologywebpages/Moulds/Scedosporium.html
http://thunderhouse4-yuri.blogspot.com/2012/10/scedosporium-prolificans.html