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29-03-2024 09:21

Michel Hairaud Michel Hairaud

Bonjour, Je recherche la description du genre Mac

26-03-2024 17:14

Juuso Äikäs

These little black ascos were growing on a dead, d

28-07-2011 18:31

Alex Akulov Alex Akulov

Dear FriendsToday I made the pdf file of Velenovsk

27-03-2024 22:53

Charles Aron Charles Aron

Hi All,Recently I came across these minute, setose

25-03-2024 13:41

B Shelbourne B Shelbourne

• Hyaloscyphaceae (no VBs), Hyaloscypha: Macro a

25-03-2024 21:27

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde, March 2024

26-03-2024 14:37

DAVID MARCOS DAVID MARCOS

Spores (telles qu'elles apparaissent à l'intérie

24-03-2024 08:27

Thierry Blondelle Thierry Blondelle

HiOn Hedera helix fallen branchEcological habitat:

26-03-2024 11:06

michel bertrand

Bonjour, Malgré de nombreuses recherches, je n'a

25-03-2024 14:08

Ursula Kozik

Bonjour et bonne journée, On m'a envoyé une tro

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Is it Gliocladium ???
Stephen Mifsud, 23-10-2020 12:02
Stephen Mifsud

Hi, I found small white-cream colonies on decaying seeds of Washingtonia, which I cultured. The resulting colonies where fluffy ash-white with a widespread maube-violet central zone. Under the microscope, the first thing that was stricking was the irregular size of the spores, ranging from 5 to 10um long. Spore dimensions from pixmetere:
(5.7) 6.4 - 9.6 (10.4) × (3.3) 3.4 - 4.9 (5.9) µm
Q = (1.5) 1.6 - 2.2 (2.7) ; N = 27
Me = 8 × 4.2 µm ; Qe = 1.9


The spores were terminal, solitary on undifferentiated hyphae, similar to the mycelium, but sometimes they appeared to be in small clusters, and occassionally I saw a penicillate arrangement on very long conidiophore.


I dont know if this is some irregular or not well known Gliocladium sp.

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David Malloch, 23-10-2020 19:59
David Malloch
Re : Is it Gliocladium ???
Hello Stephen,

Perhaps this is a member of the Microascaceae. The habitat on decomposing seeds would be common for that family.  The conidiophores and hyphae remind me of Scedosporium or perhaps an early stage of a Graphium. Watch for ascomata of Petriella or Pseudallescheria in your cultures.

David
Stephen Mifsud, 23-10-2020 21:14
Stephen Mifsud
Re : Is it Gliocladium ???
Thank you David, I will continue my investigatoion in that direction you kindly suggested and I think you are correct. There are many things which are matching, habitat, habit, variety of conidiophores (short, long, synnematous), spores with truncate bases and if I understood the annelid conidiophores with reference to wrinkled sides (seen in one of my images without congo red). The cottony colony with a dark centre is also diagnostic.

This genus is rather complex forming different reproductive structures - myself I was confused if I have a contamination of two species.

I examined my cultures by taking a film of mycelia from the surface with cellophane tape. I will investigate further down. Pure subcultures are growing fine too. Seems to be a hard one re determination to species! 

Thank you for replying and sharing your knowledege!


Important references

http://website.nbm-mnb.ca/mycologywebpages/Moulds/Scedosporium.html
http://thunderhouse4-yuri.blogspot.com/2012/10/scedosporium-prolificans.html