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Hi to everybody.I would appreciate any ideas about

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Anthracobia
Zuzana Sochorová (Egertová), 07-10-2020 13:36
Zuzana Sochorová (Egertová)Hello,
I have here a collection of Anthracobia from a fireplace in South Bohemia, the Czech Republic. I think it is A. tristis (the shape of spores seems to be enough cylindrical to me), but I almost don´t collect this genus, so I prefer to consult the determination.
Ascospores measure (15.4) 16.5-18.4 (18.7) x (7.9) 8-8.7 micrometers, Q = (1.8) 1.9-2.2 (2.3).

Hairs are brown, in fascicles, it wasn´t possible to observe them individually. The apical cell is up to 46 micrometers long and 10-17 micrometers wide.
Zuzana
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Zuzana Sochorová (Egertová), 07-10-2020 13:40
Zuzana Sochorová (Egertová)
Re : Anthracobia
Some more photos.
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Mirek Gryc, 07-10-2020 14:10
Re : Anthracobia
Hi Zuzana
As far as I know, only this species is said to have cylindrical spores.
Could you please check if the paraphyses content changes color in Lugol?
http://grzyby-pk.pl/gat_a/imgduze/anthracobia_tristis15.jpg
Mirek
Zuzana Sochorová (Egertová), 07-10-2020 18:25
Zuzana Sochorová (Egertová)
Re : Anthracobia
Hi Mirek,
thank you. The collection is already dry, but I can test the reaction later (although I think it doesn´t help in distinguishing the species too much, as it´s normal that carotenoid pigment turn green).
Z.
Lothar Krieglsteiner, 07-10-2020 20:16
Lothar Krieglsteiner
Re : Anthracobia
Hi Mirek,
doesn`t IKI change the color in all orange operculates? (carotenoids changing from orange to greenish). I thought I once read this, but at the moment I do not find it.
Best, Lothar
Mirek Gryc, 08-10-2020 08:51
Re : Anthracobia
Hi to all
Until this year, I thought similarly, but now I have some doubts. I thought this reaction had escaped my attention. Previously, I only noticed it in A. tristis and I was not sure if it occurs in other species. This year I found other collections, including:
- A. melaloma; http://www.grzyby-pk.pl/gat_a/gat_anthracobia_melaloma.php
- A. nitida: http://www.grzyby-pk.pl/gat_a/gat_anthracobia_nitida.php
In these collections such a reaction did not occur, and therefore, out of curiosity, I asked for such a test with Zuzana fruiting bodies.
It is important that I tested in Lugol, maybe it is different in IKI ???
I don't have IKI at the moment so I haven't checked it!
greetings
Mirek
Lothar Krieglsteiner, 09-10-2020 03:05
Lothar Krieglsteiner
Re : Anthracobia
Hello Mirek,
IKI is Lugol (or Baral) - it maybe different in Melzer (with chloral hydrate added, which is letal). I thought it would not make a difference here (?).
Best, Lothar
Mirek Gryc, 09-10-2020 09:07
Re : Anthracobia
Hi Lothar.
Man learns all his life and still makes mistakes, but thanks to kind people he gets out of error faster :)
I also thought that the reaction of the paraphyses content was a common feature for this kind, but as you can see, there are collections that are not affected by this. I don't know if I will meet any Anhracobia this year, but I will definitely be checking if this reaction occurs in other species.
greetings
Mirek
Lothar Krieglsteiner, 09-10-2020 10:13
Lothar Krieglsteiner
Re : Anthracobia
Hello Mirek,
"I don't know if I will meet any Anhracobia this year, but I will definitely be checking if this reaction occurs in other species."
So will I :-)
"Man learns all his life and still makes mistakes, but thanks to kind people he gets out of error faster :)"
The same is correct for me ...
Best regards, Lothar
Zuzana Sochorová (Egertová), 09-10-2020 18:50
Zuzana Sochorová (Egertová)
Re : Anthracobia
Actually, the reaction in Melzer is used in the key to Anthracobia in Nordic Macromycetes, but I was told it's not a good character...I don't know, maybe it is :-)
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Viktorie Halasu, 09-10-2020 21:56
Viktorie Halasu
Re : Anthracobia
Hello all, 

to add to the chaos: Larsen in his thesis about Anthracobia writes "paraphyses ... filled with small orange granules which turn green in iodine" at all species: macrocystis, melaloma (=intermixta, =maurilabra), nitida, tristis (=muelleri). Even A. subatra has "paraphyses ... brown-walled, turning green in iodine" (without mentioning any VBs). 

I would be interested in two things in this genus: Is there difference in iodine reaction between yellow carotenoids and those dark orange to red? Is the presence of yellow vs. red carotenoids a constant character of a species? Alas I have too little experience with the genus to know the answers.

Viktorie