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Capitotricha from California
Alan Rockefeller, 03-07-2018 13:21
Alan RockefellerI sequenced this little montane asco from a conifer stick that I expected to turn out to be Lachnum - However the ITS sequence came back and it appears to be a Capitotricha.

I just did a bit of microscopy on it.   Macro and micro photos are available at https://mushroomobserver.org/317633. />
The microscopy is all in Melzer's, and everything is inamyloid.    Spores are very long and narrow.

ITS sequence:  
https://www.ncbi.nlm.nih.gov/nuccore/MH511099

Any thoughts on this?
  • message #53990
Hans-Otto Baral, 03-07-2018 17:19
Hans-Otto Baral
Re : Capitotricha from California
Hi Alan

very interesting case. The hair morphology is clearly that of a Capitotricha. Did you see any crystals on the hairs? Did you measure the spores? They look quite normal for the genus. I do not understand your statement of very long and narrow spores, are you sure you put the right ascus and spores photos?

A pity that you mounted oin MLZ!!! Important would be to check the ascus apex in Lugol for possible hemiamyloidity (or KOH-pretreated if you don't have Lugol) and the ascus base for croziers (in KOH and/or Congo Red).

Now, your sequence belongs to "Lachnellula" cervina, which in fact falls in Capitotricha. It is a species with 48-65 x 1.5 µm large spores on Betula branch (type, Dennis 1963: 338).

You get this result when testing the ITS in the CBS database:

http://www.westerdijkinstitute.nl/Collections/BioloMICSSequences.aspx

This is a find from USA Oregon, on Alnus rubra dead twig.

There is only 1 nt difference. This suggests that your sequence belongs to a fungus with very long, filiform spores.

But I must say I have  a little doubt because C. bicolor on Alnus viridis (LT904858) is very close to it.

Zotto
Hans-Otto Baral, 07-07-2018 21:47
Hans-Otto Baral
Re : Capitotricha from California
Hi Alan
I wonder if you could find out anything about this case.
Zotto