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Jason Karakehian
The entire run of Mycotaxon is now available throu
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Mick PeerdemanHi all,One of my earliest microscopy attempts, so
Black with white hairs 280117 361
Miguel Ángel Ribes,
01-02-2017 01:07
Good nightThis small (500-600 µm) black ball with white hairs was growing at the beach, 1 m, at Thymelaea hirsuta branches.
Mature spores slightly brown, with 3 septa, (22,5) 25,2 - 28,5 (29,6) × (7,9) 8,2 - 9,7 (10) µm; Q = (2,4) 2,8 - 3,2 (3,3); N = 35; Me = 26,9 × 9 µm ; Qe = 3.
Asci 8-spored, with croziers, IKI-, (90,7) 98 - 124,6 (133,4) × (14,8) 17,3 - 20 (21,7) µm; N = 34; Me = 113 × 18,4 µm
Ectal excipulum brown with textura angularis. Sorry, imposible to make a transversal section.
Paraphysis slightly moniliform.
White hairs with refringent and thick wall, 150-200 µm.
Thank you in advance.
Miguel Á. Ribes
Andrew N. Miller,
01-02-2017 01:18
Re : Black with white hairs 280117 361
Helminthosphaeria cf. ludens, but the hairs should be darker and many of the asci should have 4 mature spores.
http://wwx.inhs.illinois.edu/files/5714/0191/3047/Helminthosphaeriaceae_Miller_et_al_Mycologia2014.pdf
Andy
http://wwx.inhs.illinois.edu/files/5714/0191/3047/Helminthosphaeriaceae_Miller_et_al_Mycologia2014.pdf
Andy
Miguel Ángel Ribes,
01-02-2017 08:00
Re : Black with white hairs 280117 361
Thank you Andrew
This night at home I will check all my asci pictures, but I remember all of them wiht 8 spores. About hairs, none not white; all old apos, when they loose the hairs, the few of them that remain are white too.
None similar species?
Best wishes.
This night at home I will check all my asci pictures, but I remember all of them wiht 8 spores. About hairs, none not white; all old apos, when they loose the hairs, the few of them that remain are white too.
None similar species?
Best wishes.
Andrew N. Miller,
01-02-2017 16:20
Re : Black with white hairs 280117 361
This is the closest species that I know of. All others have shorter ascospores. See my key. Lots of cryptic species in the Helminthosphaeriaceae.
Best,
Andy
Best,
Andy
Miguel Ángel Ribes,
01-02-2017 22:32
Re : Black with white hairs 280117 361
Hi Andrew
Yes, with your key I arrive to H. ludens, but my spores are a little bigger, hairs are white and asci have 8 spores, inmature and mature (pictures attached).
No doubt about Helmintosphaeria? I said this because my ascomata is not hard and carbonaceus, it is soft.
Thank's a lot.
Yes, with your key I arrive to H. ludens, but my spores are a little bigger, hairs are white and asci have 8 spores, inmature and mature (pictures attached).
No doubt about Helmintosphaeria? I said this because my ascomata is not hard and carbonaceus, it is soft.
Thank's a lot.
Andrew N. Miller,
01-02-2017 22:42
Re : Black with white hairs 280117 361
If you have 20-30 ascomata, send them to me and I can sequence this species and see what the DNA says.
Best,
Andy
Best,
Andy
Miguel Ángel Ribes,
02-02-2017 21:12
Re : Black with white hairs 280117 361
No, no more than 8-10 ascomata, sorry, but if you want I could send all to you.
Best wishes.
Best wishes.
Andrew N. Miller,
02-02-2017 21:17
Re : Black with white hairs 280117 361
Bummer... but this is typical for Helminthosphaeriaceae, which is why only insane people work on this group - ha!
No need to send. I do not think I can get a good sequence from only a few ascoamta, and these things do not germinate in culture.
Best,
Andy
No need to send. I do not think I can get a good sequence from only a few ascoamta, and these things do not germinate in culture.
Best,
Andy
Miguel Ángel Ribes,
02-02-2017 21:46
Re : Black with white hairs 280117 361
Jejejeje.
The collection was made at 500 km of my home, difficult to pick up again. Next year perhaps...
Thank you again.
The collection was made at 500 km of my home, difficult to pick up again. Next year perhaps...
Thank you again.
Miguel Ángel Ribes,
07-02-2017 18:47
Re : Black with white hairs 280117 361
Hello Andy
I have check all the branch slowly and I have count already 30 ascomatas (someone have no hairs, so difficult to see). I could sent you all my material if you give me your adress.
It is now in fresh condition, do you prefer I sent it fresh or dried?
Thank you.
I have check all the branch slowly and I have count already 30 ascomatas (someone have no hairs, so difficult to see). I could sent you all my material if you give me your adress.
It is now in fresh condition, do you prefer I sent it fresh or dried?
Thank you.
Andrew N. Miller,
07-02-2017 18:59
Re : Black with white hairs 280117 361
Please send slowy dried (not too much heat) to:
Dr. Andrew Miller
University of Illinois
Illinois Natural History Survey
1816 South Oak Street
Champaign, IL 61820-6970
phone: (217) 244-0439
Best,
Andy
Dr. Andrew Miller
University of Illinois
Illinois Natural History Survey
1816 South Oak Street
Champaign, IL 61820-6970
phone: (217) 244-0439
Best,
Andy
Miguel Ángel Ribes,
07-02-2017 19:13
Re : Black with white hairs 280117 361
Ok, Andrew, I will dried it at home temperature.
Best wishes.
Best wishes.
Andrew N. Miller,
07-02-2017 19:15
Re : Black with white hairs 280117 361
Cool
Andy
Andy