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13-12-2025 17:26

Buckwheat Pete

Hello everyone,I have a rather interesting ascomyc

13-12-2025 11:58

Diaporthe oncostoma?

Mirek Gryc

HiSupposedly this is a species that occurs quite o

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Similar to Pyrenopeziza deuncinata?

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Hypobryon heterotropum

Andgelo Mombert

Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

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Ascomycet mit Sammelfruchtkörper.

Arnold Büschlen

Hallo, ich habe in einer Moos-Aufsammlung (epiphy

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Hymenoscyphus phyllogenus

Mark Stevens

"Hello everyone,I'm relatively new to microscopy (

08-12-2025 18:59

aff. Tricharina?

Lothar Krieglsteiner

Lothar Krieglsteiner

.. found by a seminar-participant, I do not know t

08-12-2025 21:18

Buckwheat Pete

Hello everyone, Is it possible to at least approx

07-12-2025 17:43

Malcolm Greaves

Malcolm Greaves

This Helvella was in mixed woodland. Uniform cupul

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possibly related to Melanomma - on Abies

Lothar Krieglsteiner

Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

Black disco on Larix decidua

Adam Polhorský, 31-05-2016 15:20

Hello everyone,

black apothecia(1,1mm) with remnants of a margin growing on a weathered dry stump of Larix decidua.

Asci: IKI-, 8-spored, Crozier+ (not sure)
Spores: 16-30 x 2,5-4um, with many small guttules, multicelled- up to 10.
Paraphyses: thread-like

In a field i thougt it would be something from Rhytismatales, but microscopy excluded my candidates.
Thanks for the advice

Adam

Hans-Otto Baral, 31-05-2016 15:29

Hans-Otto Baral

Re : Black disco on Larix decidua

My guess would have been Pragmpora amphibola, but that species has apos 0.3-0.8 mm, paraphyses with refractive intercellular substance, and perhaps shorter spores. Could you please correct your spore data, does it mean 16-30 or 30-36?

Adam Polhorský, 31-05-2016 16:32

Re : Black disco on Larix decidua

U are right about the apo size, measured the largest one with the microscope - 1,1 mm. but the spores are truly 16-30um (the free, released ones)
I looked at Pragmopora before but excluded it due to spore size/ septation, paraphyses are too thin 1,2um- maybe theres some content but i cannot see it w my setup, also the margin is different? atypical maybe... and substrate is different. Maybe within variation or closely related species?

Hans-Otto Baral, 31-05-2016 16:51

Hans-Otto Baral

Re : Black disco on Larix decidua

I think the oleaginous matter between the paraphyses (not within) is a valuable character, though not only in Pragmopora. I think you shoud see that if it is there. Best is to use water as medium.
I attach a drawing of mine and a photo by Luis Quijada.

Adam Polhorský, 31-05-2016 18:01

Re : Black disco on Larix decidua

I dont see it Zotto, it looks easily observable but i see nothing, only asci and paraphyses nothing inbetween.
Maybe i can get it sequenced, but there´s no Pragmopora in genbank to compare with.

Hans-Otto Baral, 31-05-2016 21:06

Hans-Otto Baral

Re : Black disco on Larix decidua

It may be something else. Yes, it is not in GenBank, but I should get an inofficial sequence from our culture (ITS and perhaps LSU). So sequencing would be very important in this group.

Adam Polhorský, 31-05-2016 21:14

Re : Black disco on Larix decidua

OK Zotto, thank you for helping,
i will let u know when i make progress with this collection.

Adam