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Cheilymenia puzzle
Chris Yeates,
15-04-2015 23:48
Bonsoir tousI am having problems with a Cheilymenia recently collected on a pile of mixed dung and straw. It was growing in dense patches and was of a rich orange-red colour.
Apothecia to 2mm diameter
Asci 8-spored, I think with croziers.
Ascospores smooth 16.7-18 x 9.6-10.6µm
Paraphyses with carotenoid pigmentation, slightly enlarged at apices; also with occasional swellings along their length (see image).
Marginal hairs not very evident under a x10 handlens; sub. micr. brown, thick-walled, and somewhat rooting, non-stellate; up to 240µm long by average 18-20µm at their broadest point.
Using Doveri I keep coming to Cheilymenia rubra, but my experience with Cheilymenia is very limited so any suggestions would be very welcome.
Cordialement
Chris
Nicolas VAN VOOREN,
16-04-2015 08:27
Re : Cheilymenia puzzle
Hi Chris.
You need to observe ejected ascospores in Cotton Blue to check if they are really smooth.
You need to observe ejected ascospores in Cotton Blue to check if they are really smooth.
Chris Yeates,
16-04-2015 21:40
Re : Cheilymenia puzzle
Salut Nicolas
when I made the original post the fungus was not ejecting mature spores. Now it has and the spores are definitely ornamented, with very low warts which are scarcely visible in profile. These images were made at x1000 under oil, and using Nomarski DIC illumination. A series of optical 'slices' was made in each case and then stacked using Zerene Stacker software. The images were then edited to remove any artefacts created by the stacking procedure, to ensure that the resulting images were as close as possible to what I observed down the microscope.
Had I been unsure as to the presence of ornamentation I would have stained with CB, but I always prefer to deal with living material whenever I can. As well as the spore ornamentation a gel sheath was observed, both on spores still in the ascus and on ejected ones as seen here. I also include a photograph showing the growth of the fungus in the field; it seemed to favour areas of quite rotted (cattle? possibly horse) dung which was often colonised by simple algal growth.
Amitiés
Chris
when I made the original post the fungus was not ejecting mature spores. Now it has and the spores are definitely ornamented, with very low warts which are scarcely visible in profile. These images were made at x1000 under oil, and using Nomarski DIC illumination. A series of optical 'slices' was made in each case and then stacked using Zerene Stacker software. The images were then edited to remove any artefacts created by the stacking procedure, to ensure that the resulting images were as close as possible to what I observed down the microscope.
Had I been unsure as to the presence of ornamentation I would have stained with CB, but I always prefer to deal with living material whenever I can. As well as the spore ornamentation a gel sheath was observed, both on spores still in the ascus and on ejected ones as seen here. I also include a photograph showing the growth of the fungus in the field; it seemed to favour areas of quite rotted (cattle? possibly horse) dung which was often colonised by simple algal growth.
Amitiés
Chris
Michel Delpont,
17-04-2015 11:36
Re : Cheilymenia puzzle
Hello Criss.
Your fungus seems to match C.rubra; may be the erubescens variety but to check. Think also compare with C.coprinaria normally has longer hair.
The substrate is in my opinion the old cow dung.
Regards.
Michel.
Your fungus seems to match C.rubra; may be the erubescens variety but to check. Think also compare with C.coprinaria normally has longer hair.
The substrate is in my opinion the old cow dung.
Regards.
Michel.
Chris Yeates,
17-04-2015 22:57
Re : Cheilymenia puzzle
Merci beaucoup Michel.
amitiés
Chris
amitiés
Chris