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Mollisia
Garcia Susana, 09-04-2014 09:05
Hello

I collected this mollisia growing on wood.


data:
Size up to 1.5mm diameter.
KOH + (yellow)
Size Asci 73-81 x 6.5-7.5um.
Asci IKI + (blue)
septate spores


Followings the key mollisias, but did not get to determine the species.
Can anyone help?


Thank you.
Susana

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Hans-Otto Baral, 09-04-2014 09:32
Hans-Otto Baral
Re : Mollisia
Hi Susana

did you see also living asci? It seems that the spores are septate already prior to discharge, because all of them are septate, but this should be ascertained. Your ascus measurements refer to the dead state?

Zotto
Garcia Susana, 09-04-2014 11:35
Re : Mollisia
Hello Zotto

I send some more picture. Not sure if they are alive or dead.
All asci I observed, had septate spores.
I will try to make new microscopic observations.
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Hans-Otto Baral, 09-04-2014 11:48
Hans-Otto Baral
Re : Mollisia
Yes! I see two living asci and they contain septate spores.

Several collections with septate spres are in my documents (cytisi, atlantica), but all are KOH-. Did you take a photo of the yellow reaction to be sure?
Garcia Susana, 09-04-2014 20:26
Re : Mollisia
Hello Zotto

I have been tested for KOH again and I think is positive. I see a tenuous yellow halo around the apothecium. It is difficult to photograph, I think you can see in the photo I send.


What do you think?

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Hans-Otto Baral, 09-04-2014 20:35
Hans-Otto Baral
Re : Mollisia
I know it is difficult to get the yellow colour visible on digiphotos. At least it cannot be strong in your case.

I have not seen much colletions of this group of septate-spored lignicolous species, so I have no clear concept. Maybe Luis Quijada gets more progress in the matter, he also does sequencing on these Mollisias.

Was the wood lying on the moist ground? Any idea of the host genus?

Zotto
Garcia Susana, 09-04-2014 20:57
Re : Mollisia
It was on a old picket fence, and I think it may be oak.

Thanks,
Susana