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Hymenoscyphus laetus
Miguel Ángel Ribes,
18-03-2011 10:32
Good morningThis Hymenoscyphus is from Fuente del Paco, Huesca, Pyrenees, in a mixed forest of Fagus sylvaticus and Abies alba, 2-4 mm broad.
Spores (1000x, water, fresh material)
(13.4) 14.9 - 18.0 (23.9) x (3.9) 4.5 - 5.4 (5.8) µm
Q = (2.6) 2.9 - 3.8 (4.8) ; N = 67
Me = 16.5 x 4.9 µm ; Qe = 3.4
Are you agree with H. laetus?
Thank you.
Miguel Á. Ribes
Miguel Ángel Ribes,
18-03-2011 10:33
Re:Hymenoscyphus laetus
Asci with croziers and apex blue with IKI
Miguel Ángel Ribes,
18-03-2011 10:34
Re:Hymenoscyphus laetus
Spores in water
Miguel Ángel Ribes,
18-03-2011 10:35
Re:Hymenoscyphus laetus
Paraphysis plenty of guttules
Miguel Ángel Ribes,
18-03-2011 10:36
Re:Hymenoscyphus laetus
And finally ectal excipulum, I think with prismatic texture.
Thank you
Miguel Á. Ribes
Thank you
Miguel Á. Ribes
Hans-Otto Baral,
18-03-2011 14:23
Re:Hymenoscyphus laetus
Did you find this in autumn? The substrate would be interesting: Abies and Fagus are easily distiinguished when breaking the twig (Fagus with distinct radial rays).
This is H. calyculus. H. laetus has very short stipes and the spores are completely homopolar (the spores on your pic have an upper and lower end, though not very distinct: slightly scutuloid).
Zotto
This is H. calyculus. H. laetus has very short stipes and the spores are completely homopolar (the spores on your pic have an upper and lower end, though not very distinct: slightly scutuloid).
Zotto
Miguel Ángel Ribes,
18-03-2011 14:41
Re:Hymenoscyphus laetus
It is dated on 11-Oct-2010. I wiil check the wood, I think it is Fagus.
Perhaps in this photo the scutuloid spres are more clear.
Thank you Zotto
Miguel Á. Ribes
Perhaps in this photo the scutuloid spres are more clear.
Thank you Zotto
Miguel Á. Ribes
Hans-Otto Baral,
18-03-2011 22:27
Re:Hymenoscyphus laetus
yes, that's fine! And also the sheath bis visible here that detaches from the spores.
H. laetus is restricted to calcareous rivulets where it grows on wet wood lying for a long time in the running but shallow water.
Zotto
H. laetus is restricted to calcareous rivulets where it grows on wet wood lying for a long time in the running but shallow water.
Zotto
Raúl Tena Lahoz,
19-03-2011 19:10
Re:Hymenoscyphus laetus
Hi Miguel Ángel & Zotto
Here two links with my pics about Hymenoscyphus laetus:
https://picasaweb.google.com/raultenalahoz/20101004HymenoscyphusLaetusPitarque?authkey=Gv1sRgCL2azsT2nub81QE&feat=directlink
https://picasaweb.google.com/raultenalahoz/LaetusPitarque?authkey=Gv1sRgCNLw2_qprur3Zg&feat=directlink
But I saw a strange reaction in some tips of the asci. Not all reacted like that but it was striking to see some half red-half blue! Maybe due to the soaked habitat?
Also some carotenoids guttules in subhymenium.
Raúl
Here two links with my pics about Hymenoscyphus laetus:
https://picasaweb.google.com/raultenalahoz/20101004HymenoscyphusLaetusPitarque?authkey=Gv1sRgCL2azsT2nub81QE&feat=directlink
https://picasaweb.google.com/raultenalahoz/LaetusPitarque?authkey=Gv1sRgCNLw2_qprur3Zg&feat=directlink
But I saw a strange reaction in some tips of the asci. Not all reacted like that but it was striking to see some half red-half blue! Maybe due to the soaked habitat?
Also some carotenoids guttules in subhymenium.
Raúl
Hans-Otto Baral,
19-03-2011 23:14
Re:Hymenoscyphus laetus
ah, you see the upper ring reddish. Did you also test KOH-pretreatment? Maybe the upper ring turns then also blue?
I must correct myself, I think Miguels colection is H. subferrugineus. I once thought that H. calyculus is frequent, but I actually did not see it for a long time. It has larger spores with larger LBs inside, but I admit the distinction to subferrugineus is not really clear.
Zott
I must correct myself, I think Miguels colection is H. subferrugineus. I once thought that H. calyculus is frequent, but I actually did not see it for a long time. It has larger spores with larger LBs inside, but I admit the distinction to subferrugineus is not really clear.
Zott
Raúl Tena Lahoz,
20-03-2011 15:05
Re:Hymenoscyphus laetus
Thanks Zotto
Yes, the upper ring red (hemiamyloid) and more down blue (euamyloid). Not tested KOH-pretreatment. I will check it when I get it again (I think it is common in my area).
Should I see then (after KOH-pretreatment) two types of blue if it is an hemiamyloid reaction? If upper part remains unstained (also after KOH-pretreatment, as in the attached image), then no hemiamyloid reaction?, so red colour is maybe then because of my IKI composition?
I attach an image of Hymenoscyphus caudatus asci (find on Populus nigra leaves) in IKI after KOH-pretreatment.
Raúl
Yes, the upper ring red (hemiamyloid) and more down blue (euamyloid). Not tested KOH-pretreatment. I will check it when I get it again (I think it is common in my area).
Should I see then (after KOH-pretreatment) two types of blue if it is an hemiamyloid reaction? If upper part remains unstained (also after KOH-pretreatment, as in the attached image), then no hemiamyloid reaction?, so red colour is maybe then because of my IKI composition?
I attach an image of Hymenoscyphus caudatus asci (find on Populus nigra leaves) in IKI after KOH-pretreatment.
Raúl
Hans-Otto Baral,
20-03-2011 17:59
Re:Hymenoscyphus laetus
I rarely saw such differences between blue and red in a single ring. If so, this diference will change to uniformly blue after KOH. If the upper ring does not get blue thereby, it s something else I do not understand.
Zotto
Zotto
Raúl Tena Lahoz,
20-03-2011 18:42
Re:Hymenoscyphus laetus
Ok, thanks again.
Raúl
Raúl
Miguel Ángel Ribes,
21-03-2011 00:58
Re:Hymenoscyphus laetus
Hi Zotto, Raúl
I did not picked up this collection, but I know the place, and it is not so wet like Raul's collection of H. laetus.
I think spores of my collection are closer to your plates of H. subferrugineus than H. calyculus, thank you Zotto, so H. subferrugineus, by the moment.
Thank you,
I did not picked up this collection, but I know the place, and it is not so wet like Raul's collection of H. laetus.
I think spores of my collection are closer to your plates of H. subferrugineus than H. calyculus, thank you Zotto, so H. subferrugineus, by the moment.
Thank you,