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10-04-2020 19:01

Miguel Ãngel Ribes Miguel Ángel Ribes

Good afternoonThis small Mollisia, 1-2 mm, from Mo

04-04-2020 19:03

Andgelo Mombert Andgelo Mombert

Bonsoir, Sur branche morte aérienne de Crataegus

04-04-2020 15:13

Nicolas Schwab

Bonjour, Skrede et al. 2017, 2020 considèrent He

10-04-2020 20:15

Viktorie Halasu Viktorie Halasu

Hello,I've found this Orbilia on wet decorticated

10-04-2020 19:11

Lepista Zacarias

Dear all,I would like to have your help in the ide

07-04-2020 11:03

Malcolm  Greaves Malcolm Greaves

Has anyone got a key to Pososordaria or informatio

10-04-2020 16:28

Rubén Martínez-Gil Rubén Martínez-Gil

Hola a todos. Tengo esta recolecta del verano pas

08-04-2020 11:36

Garcia Susana

Hola, Pseudotecios muy pequeños, unos 120um de d

09-04-2020 19:51

Miguel Ãngel Ribes Miguel Ángel Ribes

Good evening guysI found this small apos (1,5-2 mm

07-04-2020 23:11

Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et à tous,Trouvé hier ce que j

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Mollisia 081219 077 on Prunus lusitanica
Miguel Ángel Ribes, 10-04-2020 19:01
Miguel Ãngel RibesGood afternoon

This small Mollisia, 1-2 mm, from Morocco was growing on Prunus lusitanica branch. Ectal excipulum brown from base to margin, with textura angularis, with somewhat more elongated cells at the margin, but without forming cylindrical cells. Medullar excipulum with textura intricata, hyaline too. Subhimenium also hyaline. Paraphysis with 1 single BV that occupies the entire terminal cell, KOH -. Asci octosporic, with croziers, euamiloyd. Cylindrical spores with small LBs at the ends, (8.1) 8.8 - 10 (11.9) × (2.5) 2.6 - 3 (3.1) µm; Q = (2.9) 3.1 - 3.8 (4.2); N = 45; Me = 9.4 × 2.8 µm; Qe = 3.4.

With these micro I come to M. melaleuca, but I see longer spores in the biblio than mine. Other nearby species that I arrive at are M. peruni, M. rivularis and M. cinerea.

Thank you in advance.
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Hans-Otto Baral, 10-04-2020 22:00
Hans-Otto Baral
Re : Mollisia 081219 077 on Prunus lusitanica
The asci and paraphyses are too small on these pics, so I wasn't sure about the VBs. I saw only a few short ones, so I thought a Pyrenopehziza. But you are probably right. This could be something of the Phialocephala clade, like P. oblonga.

Zotto
Miguel Ángel Ribes, 11-04-2020 11:51
Miguel Ãngel Ribes
Re : Mollisia 081219 077 on Prunus lusitanica
Here are details about Asci and Paraphysis.

Asci are (74) 80,1 - 91,4 (96,3) × (6,3) 6,5 - 7,4 (7,6) µm N = 26; Me = 86,2 × 6,8 µm ; Qe = 12,6
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Hans-Otto Baral, 11-04-2020 12:42
Hans-Otto Baral
Re : Mollisia 081219 077 on Prunus lusitanica
Now it is clear. Do you have Tanney et al. 2016 about Phialocephala/Mollisia? If not I will send you (4 MB). A very detailed study.
Mirek Gryc, 11-04-2020 13:20
Re : Mollisia 081219 077 on Prunus lusitanica
Hello everyone.

Zotto, do you mean that?


 


Is there any other work?
best regards
Mirek

Martin Bemmann, 11-04-2020 13:39
Martin Bemmann
Re : Mollisia 081219 077 on Prunus lusitanica
Miguel Ángel Ribes, 11-04-2020 13:52
Miguel Ãngel Ribes
Re : Mollisia 081219 077 on Prunus lusitanica
Thank you Zotto,Mirek and Martin

With Tanney et al 2016 I think it fits Ok with P. oblonga, although my spores are a little smaller ((8.1) 8.8 - 10 (11.9) × (2.5) 2.6 - 3 (3.1) µm; Q = (2.9) 3.1 - 3.8 (4.2); N = 45; Me = 9.4 × 2.8 µm; Qe = 3.4) than Tanney's ((8–)9–11.5(–17) x (2.5–)3–3.5(–4) mm).

But I do not understand this in the description of Tanney: "pore amyloid in Melzer's reagent or Lugol's solution with 5% KOH pretreatment (hemiamyloid), protoplasm turning brick red (7D7) in Lugol's solution". In the pictures I do not see the hemiamyloid reaction of asci pore.

My asci pore is euamyloid and the protoplasm turning brick red in IKI.

Miguel Ángel Ribes, 11-04-2020 13:56
Miguel Ãngel Ribes
Re : Mollisia 081219 077 on Prunus lusitanica
Now I am reading that all species in Tanney 2016 are hemiamyloid.
Hans-Otto Baral, 11-04-2020 17:30
Hans-Otto Baral
Re : Mollisia 081219 077 on Prunus lusitanica
I think Joey Tanney does not well understand hemiamyloidity. I told him this mistake some time ago, and now in the new paper (link of Martin) it reads: pore amyloid in Melzer's reagent or Lugol's solution with 5 % KOH pretreatment (euamyloid). - So I explained him that euamyloidity cannot be tested merely by iodine after KOH. I hope this will be corrected. I actually saw this articele only thanks Researchgate, and because it is a preview, I started reviewing it. This version is still without plates and trees.

Phialocephala oblonga is a choice, but I feel a little unable to identify these species without anamorph or DNA. Perhaps some time I will learn some minute differences, not sure.

Zotto
Miguel Ángel Ribes, 11-04-2020 18:07
Miguel Ãngel Ribes
Re : Mollisia 081219 077 on Prunus lusitanica
Ok, now I understand it. I was already beginning to doubt if I was wrong with the hemiamyloid reaction.

Phialocephala aff. oblonga, then.

Thanks a lot.