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29-07-2009 23:04

Nicolas VAN VOOREN Nicolas VAN VOOREN

Bonsoir. Je pense avoir récolté Pezoloma kathia

29-07-2009 15:48

Hans-Otto Baral Hans-Otto Baral

Here is a very sparse find of a minute ?Lasionectr

28-07-2009 23:56

Hans-Otto Baral Hans-Otto Baral

Peter Tobies asked me about this Hypocreaceae whic

27-07-2009 13:37

Gernot Friebes

Hello, I found this discomycet this year's spri

25-07-2009 07:15

Alain GARDIENNET Alain GARDIENNET

Bonjour, Je ne vois pas ce que peut être ce peti

24-07-2009 19:32

Edouard Evangelisti Edouard Evangelisti

Bonjour le forum ! Je sollicite votre aide pré

24-07-2009 16:48

Beñat Jeannerot Beñat Jeannerot

Bonjour à tous, Je reviens un peu dans les parag

22-07-2009 22:01

Enrique Rubio Enrique Rubio

Hi to all These gregarious ascomata grew on veget

21-07-2009 00:27

Miguel Ãngel Ribes Miguel Ángel Ribes

Hi again This another one is 1-1,5 mm, subsesil

20-07-2009 20:48

Miguel Ãngel Ribes Miguel Ángel Ribes

Hello I have this black and soft asco in a dea

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Pezoloma (or Hymenoscyphus) kathiae : confirmation
Nicolas VAN VOOREN, 29-07-2009 23:04
Nicolas VAN VOORENBonsoir.
Je pense avoir récolté Pezoloma kathiae Korf. Voici la photo macro, les éléments micros suivent.
Merci de me confirmer pour ceux qui connaissent cette espèce.
  • message #8613
Nicolas VAN VOOREN, 29-07-2009 23:08
Nicolas VAN VOOREN
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Micro :
Asques 100-115 x 8-9 µm, avec crochet, anneau apical IKI+ (bb). Paraphyses contenant de très nombreuses vacuoles réfringentes. Spores 10-14 x 4-4.5 (5) µm, avec de gros LBs et d'autres plus fins. Excipulum médullaire de textura porrecta ; excipulum ectal de textura globulosa, à cellules Ø 9-28 µm. Léger bleuissement à la base de l'apothécie en présence d'IKI.
  • message #8614
Nicolas VAN VOOREN, 29-07-2009 23:15
Nicolas VAN VOOREN
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Voici un petit montage photo, avec notamment la présence de macroconidies (je pense) à la base de l'apothécie. Je ne sais si elles sont liés à l'anamorphe de ce champignon ou à un autre, car les apothécies poussaient sur un fragment de petite branche (feuillu) dans une zone humide, fangeuse.

Lieu de récolte : bois de la Flachère, Le Bois-d'Oingt (Rhône, France), alt. 300 m, N 45°54'37" - E 4°33'57", le 25-VII-2009.
  • message #8615
Hans-Otto Baral, 29-07-2009 23:23
Hans-Otto Baral
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Hi Nicolas

yes, I think this is kathiae. The conidia might belong here because Descals or Webster described similar conidia in a fungus looking like H. imberbis. Did you measure the conidia?

Zotto
Nicolas VAN VOOREN, 29-07-2009 23:37
Nicolas VAN VOOREN
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Hi Zotto.
Yes, they can reach up to 230 µm long and 7-8 µm wide.
Richard Korf, 29-07-2009 23:48
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Certainly very like Pezoloma kathiae, but the paraphyses you draw are exceptionally broad, and I didn't see any contents such as those you illustrate. No conidia were observed by us in cultures. I don't know if Zotto has made any further exploration of these species nor whether he still believes they belong in Hymenoscyphus. Maybe there is some molecular data now?

Dick Korf
Hans-Otto Baral, 30-07-2009 00:00
Hans-Otto Baral
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Yes, we (Ricardo Galan) have molecular data on this group: the analysis was presented on the poster in 2002 by Collado et al. There, H. kathiae clusters with H. imberbis, H. vernus and Anguillospora furtiva in a distinct clade, with the next relatives Discinella terrestris and Tatraea dumbirensis. This clade is associated to the main part of Hymenoscyphus and Cyathicula.

I regret there is no Cudoniella in this tree because I think that the clade around H. imberbis/vernus could be placed in Cudoniella. There is also no Pezoloma in this tree.

Zotto
Richard Korf, 30-07-2009 01:38
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Thanks, Zotto. I suspect from what you say that these are far enough from Hymenoscyphus that you would not want to keep them there. No combination in Hymenoscyphus appears to have been made for P. kathiae. Not sure if any other Pezoloma spp. were treated in that study. I can't help wondering about those paraphyses.

Dick
Hans-Otto Baral, 30-07-2009 09:31
Hans-Otto Baral
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Dear Dick

I did not find any taxon under the name Pezoloma in this tree, but maybe there are some under another combination. P. kathiae was actually transferred to Hymenoscyphus by me: in Dimitrova & Baral 2005, Fl. Medit. 15, Checklist of Bulgarian Discomycetes. At that tiem I favoured a broad concept of the genus. On the other hand, H. imberbis was never transferred to Pezoloma, although it is hardly different except for a much lower amount of oil in the spores. Yet, I think that Pezoloma iodopedis is a synonym of H. imberbis.

About the paraphyses I forgot to write the following. Do you remember: when you published P. kathiae I compared this species with my previously unidentified collections which I considered to be conspecific. But in your drawing the paraphyses were without guttules. Since you obviously studied the specimen in the dead rehydrated state, I did not see any problems to take my collections with multiguttulate paraphyses as conspecific. The type of Pezoloma is, however, without any such guttules, even in the living state. These guttules (VBs) are a very striking feature of the living fungus. They disappear in KOH or when dried, and stain turquoise under vital staining with cresyl blue.

So I asked you about this vital character maybe 8 years ago, and I remember you went at the type locality and recollected material, and you actually found the strikingly guttulate paraphyses because now you studied the fungus directly when still fresh.

Zotto
Nicolas VAN VOOREN, 30-07-2009 13:19
Nicolas VAN VOOREN
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Thanks for all these data.
Zotto, do you have an offprint of your paper published with Dimitrova? If so, I'm interested by this article.
Richard Korf, 30-07-2009 14:38
Re:Pezoloma (or Hymenoscyphus) kathiae : confirmation
Hi, Zotto,

I forgot all that! Your combination was not picked up by Index Fungorum. Please contact Paul Kirk <p.kirk@cabi.org> and give him the full citation.

Dick