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21-04-2023 17:13

Similar to Durella suecica so Mollisia maybe?

Hardware Tony

Hardware Tony

Wonder if anyone can help here with this species w

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Similar to Durella suecica so Mollisia maybe?

Hardware Tony, 21-04-2023 17:13

Hardware Tony

Wonder if anyone can help here with this species which I thought was Durella suecica, but is IKI -, the spores are slightly larger with larger apothecia <1.0mm. Gregariously scattered under fallen branch of Pinus sylvestris with flattish surface, concolourous margins and exteriors of pale cream colour. No marginal hairs.

Ascospores: 6.53 - 8.03 x 3.31 - 3.63 µm, hyaline, non-septate, polar drops at each end, one end slightly larger both rounded.
Asci: 8-spored, Unseriate 60 - 75 x 6.85 - 7.55 µm. IKI -
Paraphyses: 10 µm ± above asci, cylindrical rounded tips, tapering up with 2-septa, filled mostly upper sections VB's, some highly refractive, some with granular exudate tips.
Croziers: Not observed.
Excipulum: Small celled subglobose texture.

with many thanks for any ideas

Hans-Otto Baral, 21-04-2023 17:28

Hans-Otto Baral

Re : Similar to Durella suecica so Mollisia maybe?

What is the substrate? Durella is excluded by the globose texture. In the dead state difficult to tell. The contents of the living paraphyses would help in this regard.

Hardware Tony, 21-04-2023 21:49

Hardware Tony

Re : Similar to Durella suecica so Mollisia maybe?

With thks Otto. On Pinus sylvestris (plus see notes above). I tried to extract as many living paraphyses as possible here, some of which may not be any longer. I hope these help. Thks Tony

Viktorie Halasu, 21-04-2023 21:56

Viktorie Halasu

Re : Similar to Durella suecica so Mollisia maybe?

Hello,
the thick ascus apex, VB+ paraphyses and spores make an impression of a Hymenoscyphus (around H. imberbis).
Viktorie

Hans-Otto Baral, 21-04-2023 22:01

Hans-Otto Baral

Re : Similar to Durella suecica so Mollisia maybe?

Sorry indeed, I overlooked the mentioned substrate. Yes, I also had an idea, something like what I call Hymenoscyphus rufescens on conifer cones (Cudoniella folder), although that has an amyloid ring.

Hardware Tony, 22-04-2023 09:03

Hardware Tony

Re : Similar to Durella suecica so Mollisia maybe?

Thks Viktorie/Otto for your findings. Mystery deepens as I re-tried and tested for amyloidity in case I had overexposed images or something and I think the result is the same as shown here although I 'tried' to find the opposite.

I could go back and obtain more material as this test exhausted all that I had left.

Thks again

Hardware Tony, 23-04-2023 17:12

Hardware Tony

Re : Similar to Durella suecica so Mollisia maybe?

Obtained more material and with Lugols and Melzers amyloidity now proven. No idea why this didn't work first time around but I see other collections (few) have shown to have had issues with visibility.

I was hoping this would make Hymenoscyphus rufescens a distinct possibility now. H. imberbis doesn't seem to fit and the excipulum seems different. Is this Hymenoscyphus or Phaeohelotium - different acceptances seen?

With thanks for your further consideration Tony

Hans-Otto Baral, 23-04-2023 21:42

Hans-Otto Baral

Re : Similar to Durella suecica so Mollisia maybe?

Wonderful, clearly of the Hymenoscyphus-type. I would not use either of the two genera but possibly Cudoniella could be a genus to use - for the future...