24-05-2015 19:53
Alessio Pierotti
I apologize now for my request.This time I do not
28-05-2015 15:24
Nicolas VAN VOOREN
An Australian colleague (Heino Lepp) submits a col
27-05-2015 22:16
Zuzana Sochorová (Egertová)
Hello,I often have troubles with determination of
24-05-2015 21:51
Eduard OsieckRecently Splanchnonema quercicola and Pleomassaria
16-05-2015 08:20
Castillo Joseba
Me envian foto desde GaliciaComo voy a estar fuera
26-05-2015 17:19
Nicolas VAN VOOREN
Bonsoir.Je cherche l'article suivant:Yao Y.J., Spo
26-05-2015 11:53
Gernot FriebesHi,I'm not sure where to place this fungus which I
26-05-2015 20:12
Quijada LuisHi all,somebody have the following manuscript:More
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
