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18-08-2025 22:59

Yanick BOULANGER

BonsoirVoici un asco récolté le 08/08/2025Comme

18-08-2025 23:15

Zoe Vélez Zoe Vélez

Hola foro, gracias por aceptar la creación de mi

18-08-2025 15:35

Lothar Krieglsteiner Lothar Krieglsteiner

.. in subarctic forest at side of small stream, ac

18-08-2025 16:01

Lothar Krieglsteiner Lothar Krieglsteiner

.. on water-soaked Betula wood lying in a small st

18-08-2025 17:52

Yanick BOULANGER

BonjourAu fil des conversations, j'ai lu que Hyalo

18-08-2025 15:17

Lothar Krieglsteiner Lothar Krieglsteiner

... on 6.7.25 in a subarctic mire near a small lak

18-08-2025 15:07

Lothar Krieglsteiner Lothar Krieglsteiner

.. 20.7.25, in subarctic habital. The liverwort i

31-12-2021 12:12

Georges Greiff

Happy New Year to All! I was hoping somebody coul

15-08-2025 12:47

Philippe PELLICIER

Bonjour, j'ai récolté cette Scutellinia au Col d

15-08-2025 21:50

Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et tous,Pourriez-vous m'aider à

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Preservation of Ascobolaceae members
Suhaib Firdous Yatoo, 18-03-2020 19:03
Suhaib  Firdous YatooWhat is the best way to preserve Ascobolaceae members, mostly the smaller ones that are less than 1mm and how to preserve them for herbarium purposes?
How to rehydrate if I want to work on them again?
(as KOH dissolves the episporium).
Hans-Otto Baral, 18-03-2020 19:05
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
The by far very best way is to study the in the fresh state. Everything else leads to minor results and includes artifacts.
Suhaib Firdous Yatoo, 19-03-2020 10:49
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
How to preserve them for herbarium?
And can we preserve the smaller ones as slides mounted in Hoyer?
Hans-Otto Baral, 19-03-2020 10:55
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
I can only recomend you to read my paper from 1992 (Mycotaxon).

Preservation in Ascobolus is not different from any other ascomycete. Drying and placing in a tight plastic bag. Hoyer's liquid I remember a bit, it is killing the cells and perhaps also dissolves structures.
Suhaib Firdous Yatoo, 19-03-2020 11:07
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
Yes, and cells seem to swell a bit.
Thank you very very much sir.