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27-04-2026 20:52

Lothar Krieglsteiner Lothar Krieglsteiner

Found on hanging tiwg of Olea europaea in dried-ou

27-04-2026 18:48

Tony Moverley

Collected 23rd April 2026, Norfolk, EnglandSwarms

27-04-2026 17:41

Lothar Krieglsteiner Lothar Krieglsteiner

.. Algarve, same leaf than the last post. The con

27-04-2026 18:05

Lothar Krieglsteiner Lothar Krieglsteiner

... still attached at standing tree. The green con

27-04-2026 17:16

Lothar Krieglsteiner Lothar Krieglsteiner

.. Algarve, moist lying.The conidiomata look like

27-04-2026 12:54

Steve Clements

Bonjour. Ce petit champignon blanc résupiné et

27-04-2026 09:59

Pauline. Penna

Bonjour Can anyone advise me on these pycnidia fo

26-04-2026 21:08

William Slosse William Slosse

Several species of Ramularia occur on Rumex that I

22-04-2026 20:54

Enrique Rubio Enrique Rubio

Hi to everybody.This Pyrenopeziza grew in moist le

25-04-2026 11:34

Louis DENY

Bonjour forumdans la clé de Zotto, L. pudicellum

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Preservation of Ascobolaceae members
Suhaib Firdous Yatoo, 18-03-2020 19:03
Suhaib  Firdous YatooWhat is the best way to preserve Ascobolaceae members, mostly the smaller ones that are less than 1mm and how to preserve them for herbarium purposes?
How to rehydrate if I want to work on them again?
(as KOH dissolves the episporium).
Hans-Otto Baral, 18-03-2020 19:05
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
The by far very best way is to study the in the fresh state. Everything else leads to minor results and includes artifacts.
Suhaib Firdous Yatoo, 19-03-2020 10:49
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
How to preserve them for herbarium?
And can we preserve the smaller ones as slides mounted in Hoyer?
Hans-Otto Baral, 19-03-2020 10:55
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
I can only recomend you to read my paper from 1992 (Mycotaxon).

Preservation in Ascobolus is not different from any other ascomycete. Drying and placing in a tight plastic bag. Hoyer's liquid I remember a bit, it is killing the cells and perhaps also dissolves structures.
Suhaib Firdous Yatoo, 19-03-2020 11:07
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
Yes, and cells seem to swell a bit.
Thank you very very much sir.