Accès membres

Mot de passe perdu? S'inscrire

27-11-2024 16:34

David Chapados David Chapados

Hi, would it be possible to get to specie with th

19-11-2024 08:27

Lothar Krieglsteiner Lothar Krieglsteiner

.. in the Algarve (Portugal) while looking for Tro

25-11-2024 15:07

Hardware Tony Hardware Tony

Only thanks to Otto's folders under Lecanorales di

23-11-2024 15:01

Lydia Koelmans

I decided to look at seriously tiny black fungal d

25-11-2024 13:00

Bernard Declercq Bernard Declercq

Dear all,This one has been collected on sooty moul

24-11-2024 09:05

Bernard Declercq Bernard Declercq

Dear all,Collected on 5 cm diam. corticated branch

23-11-2024 18:18

Marc Detollenaere Marc Detollenaere

Dear all,On a branch of a dead deciduous tree (Sal

06-08-2024 23:10

Ethan Crenson

Hi all, In NYC I found this mushroom engulfed by

21-11-2024 17:21

Malcolm  Greaves Malcolm Greaves

This Geoglossum was found in a wet meadow. 7 sept

04-11-2024 17:32

Yves Antoinette

Bonjour, je pense qu'il peut s'agir de Trichoderma

« < 68 69 70 71 72 > »
Preservation of Ascobolaceae members
Suhaib Firdous Yatoo, 18-03-2020 19:03
Suhaib  Firdous YatooWhat is the best way to preserve Ascobolaceae members, mostly the smaller ones that are less than 1mm and how to preserve them for herbarium purposes?
How to rehydrate if I want to work on them again?
(as KOH dissolves the episporium).
Hans-Otto Baral, 18-03-2020 19:05
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
The by far very best way is to study the in the fresh state. Everything else leads to minor results and includes artifacts.
Suhaib Firdous Yatoo, 19-03-2020 10:49
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
How to preserve them for herbarium?
And can we preserve the smaller ones as slides mounted in Hoyer?
Hans-Otto Baral, 19-03-2020 10:55
Hans-Otto Baral
Re : Preservation of Ascobolaceae members
I can only recomend you to read my paper from 1992 (Mycotaxon).

Preservation in Ascobolus is not different from any other ascomycete. Drying and placing in a tight plastic bag. Hoyer's liquid I remember a bit, it is killing the cells and perhaps also dissolves structures.
Suhaib Firdous Yatoo, 19-03-2020 11:07
Suhaib  Firdous Yatoo
Re : Preservation of Ascobolaceae members
Yes, and cells seem to swell a bit.
Thank you very very much sir.