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15-11-2014 11:16

Marja Pennanen

Hello forum,these have as long stipe as they are w

21-10-2015 06:12

Maren Kamke Maren Kamke

Hi everybody,the very large apothecia (up to 9 mm)

22-10-2015 18:41

Michel RIMBAUD

Bonjour,J'ai récolté cette croûte rouge grenat

22-10-2015 20:04

Ismael Wind

This asco seems to be associated with a pyrenomyce

21-10-2015 00:47

Lepista Zacarias

Lichenicolous on DegeliaThis fungus was found grow

21-10-2015 12:57

Chris Johnson

BonjourThis was found on moorland peaty soil. The

20-10-2015 11:34

Garcia Susana

HelloI found this ascomiceto growing on a branch c

20-10-2015 20:06

Marja Pennanen

Hi,I collected yesterday Urceolella pani and notic

20-10-2015 18:17

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello,this Iodophanus grew on a dung of herbivore

19-10-2015 00:03

Bometon Javier Bometon Javier

Esporas 29-33 X 7-8 um, multigutuladas, septadas e

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On Lycopodium annotinum
Marja Pennanen, 15-11-2014 11:16
Hello forum,

these have as long stipe as they are wide, about 0,3 mm.
Curiously: what is the limit between subsessile and stipate?
The spores have many droplets and are about 14-18 (-20)x3-4,5.
The asci are 4 spored, 70-100x6-9 and to my seeing IKI-.
The paraphyses are about 2 micrometers wide an may have a big vacuole at the top.
I saw one septum in some of them.

Ascomycetes on Nordic Lycopods gave me no answers.
Once again no other ideas except here to look for help with determination: Marja
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Hans-Otto Baral, 15-11-2014 11:46
Hans-Otto Baral
Re : On Lycopodium annotinum
hui, what big spores. Bad that the asci are inamyloid - so we are not sure whether it is hymenoscyphoid, or a member of Pezizellaceae (what I more suspect). Something around Allophylaria perhaps, or Calycellina.

I have nothing in my database  on Lycopodium with such large spores.

Zotto
Marja Pennanen, 15-11-2014 13:44
Re : On Lycopodium annotinum
Hi Zotto,

those are good ideas for the genus. I shall check them. I can not say, that I'm very sure of the amyloidity.
The asci seemed to be without reaction.
But the fact, that there are only 4 spores in asci is not very common and I find it interesting.


Funny, that now, that I know a bit more, my findings are no easier for me to determine. Finding new for me species is still as easy, but the species turn more uncommon.

Marja
Hans-Otto Baral, 15-11-2014 17:07
Hans-Otto Baral
Re : On Lycopodium annotinum
ah, I overlooked the tetraspory. If you have immature asci, do they also show 4 spores or 8?

Is the excipulum of text. prismatica or oblta?
Marja Pennanen, 15-11-2014 18:22
Re : On Lycopodium annotinum
Hi,

I got to look the asci again and test Iki, too. The immature asci were filled with vacuoles and the amount of spores was not a thing easy to notice.
The cells were like elongate brickwall, prismatica I suppose.

Be back later: Marja
Marja Pennanen, 15-11-2014 22:04
Re : On Lycopodium annotinum
Hi,

now. when I had time, I looked through my photos.
Here is now one with autolevels adjustment, funny colour, but vacuoles show pretty well.
There are loads of vacuoles in the immature asci, but 4 spores seems to develop in the top of the asci.
By the way, one septum is found in spores, when droplets disappear.

Marja
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Hans-Otto Baral, 15-11-2014 22:22
Hans-Otto Baral
Re : On Lycopodium annotinum
It is typical for spores rich in oil that the immature ascus is full of LB droplets. To see the immature spores is often only possible when the asci are alive. yours are too much pressed.
Marja Pennanen, 23-10-2015 15:40
Re : On Lycopodium annotinum
Hello,

I found these again. Now I can tell, that the asci are IKI+ red.
The cells get narrower towards the base.
The paraphyses seem to have VB filled tops, up to 3 wide.
The spores were 15-20x4-5 and asci 90-110x8-10, 4 spored.

Here some more photos.
The microscophy is so peculiar, that this should be very easy species to determine, after finding the genus and the right description. Or could this be a species, that is still waiting to be published?

Marja
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Hans-Otto Baral, 23-10-2015 16:14
Hans-Otto Baral
Re : On Lycopodium annotinum
Yes! And it is clearly an Allophylaria.

Zotto
Marja Pennanen, 23-10-2015 18:29
Re : On Lycopodium annotinum
Thank you!!!

Now I can write Allophylaria sp. on the specimen and the determination process  has taken a huge step further.

There seems to be 14 Allophylaria species at this moment.

Marja
Hans-Otto Baral, 23-10-2015 18:52
Hans-Otto Baral
Re : On Lycopodium annotinum
Yes, about this number.
Would be interesting to find out whether 4 spores abort or the spores are binucleate as I observed in one collection on Poaceae. Your photos look like uninucleate.