28-11-2021 11:47
Viktorie Halasu
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Calycellina on Humulus
Viktorie Halasu,
28-11-2021 11:47
Hello,I'm trying to find a name for this small yellow Calycellina. Apothecia 170-280 um in diam., growing on Humulus lupulus stalk laying in leaf detritus, in a Reynoutria stand near some trees overgrown by Humulus. Somewhat wet locality shaded from draught. Soc. Hymenoscyphus humuli. Spores are measured in KOH in asci, it wasn't ejecting spores even when fresh.
There is Michel's collection of C. flaveola on Humulus, could it be the same? I don't know if the darkening of cell walls in excipulum with age is a common character in Calycellina or not. It was collected on 22. IX. 2021 and forgotten in the fridge, photos in KOH are from today.
Thank you in advance.
Viktorie
excip-VB-0001.JPG
sp-asci-0001.JPG
para-hairs-0001.JPG
excip-dead-0001.JPG
Michel Hairaud,
28-11-2021 16:26
Re : Calycellina on Humulus
Hi Viktorie,
Your collection can be compared to mine. Your spores are a bit smaller (mine are 14-16 x 3-3,8 µm) but may be because of measurement on dead materiel ?
I did not notice the blackening of excipular celles you show.
I still tentatively name it C. humuli. It is rather common in the wet zone of Le Marais Poitevin when it's wet enough
Amitiés Michel
Your collection can be compared to mine. Your spores are a bit smaller (mine are 14-16 x 3-3,8 µm) but may be because of measurement on dead materiel ?
I did not notice the blackening of excipular celles you show.
I still tentatively name it C. humuli. It is rather common in the wet zone of Le Marais Poitevin when it's wet enough
Amitiés Michel
Hans-Otto Baral,
28-11-2021 18:28
Re : Calycellina on Humulus
There are sequences of C. flaveola from two finds of Stip, but they differ by about 4.5%. One is very close to C. ulmariae and the other very close to C. fagina. No idea what heppened there.... One was on Pteridium, the other ?also
Michel Hairaud,
28-11-2021 18:55
Re : Calycellina on Humulus
C. flaveola is different I think, and I do have the project to have this species on Humulus sequenced ...
I haven't seen Stip's samples.
Michel
I haven't seen Stip's samples.
Michel
Stip Helleman,
28-11-2021 20:19
Re : Calycellina on Humulus
I know only of one SBRH836, it is from Pteridium and there is also one C.fagina or is there a sequence extracted by Guy?
cheers,
Stip
Hans-Otto Baral,
28-11-2021 20:29
Re : Calycellina on Humulus
It is this one:
Calycellina flaveola SBRH 871, D, Saarland, Nonnweiler, Pteridium leaves, 03-VIII-16
Yes, must have done Guy, it is a comprehensive sequence.
Stip, do you have the ab1 of 836 at hand? I would like to check it regarding the few unknown positions. Then we know perhaps more exactly what's the matter here.
Zotto
Stip Helleman,
28-11-2021 20:38
Re : Calycellina on Humulus
Aha, I have not seen the sequence, der hat er extrahiert wenn wir ihn aufgesucht habe in 2016
I will send the ab1
cheers, Stip
Viktorie Halasu,
29-11-2021 16:20
Re : Calycellina on Humulus
Hello all,
thank you for this interesting discussion.
Michel: Ok, I'll file it under "C. humuli nom.prov.". Hopefully next year I will find it again and have it sequenced, this material is too sparse and too contaminated by micros. Excipular cells were hyaline when fresh, the blackening appeared after 6 weeks in the fridge (not a surprise here, I too would change color significantly after such a treatment).
Have a nice day.
Viktorie
thank you for this interesting discussion.
Michel: Ok, I'll file it under "C. humuli nom.prov.". Hopefully next year I will find it again and have it sequenced, this material is too sparse and too contaminated by micros. Excipular cells were hyaline when fresh, the blackening appeared after 6 weeks in the fridge (not a surprise here, I too would change color significantly after such a treatment).
Have a nice day.
Viktorie
Michel Hairaud,
29-11-2021 17:30
Re : Calycellina on Humulus
Please do stay outside the frige ,Viktorie !Michel