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13-09-2013 09:58

Alessio Pierotti Alessio Pierotti

can someone help me? I'm looking for these two tit

24-09-2013 22:00

Malcolm  Greaves Malcolm Greaves

HiIs anyone able to confirm that this is Saccobolu

24-09-2013 13:21

Garcia Susana

Ascocarps globose (200-215um), with a long neck (5

22-09-2013 11:36

Guy Garcia

Dear all,I search the paper where is published Lac

23-09-2013 23:49

Esquivel-Rios Eduardo

Hola todos.Esta Cookeina ha sido encontrada recien

20-09-2013 21:31

Ralph Vandiest Ralph Vandiest

Hello,I found these little white species on beech

20-09-2013 22:46

Ralph Vandiest Ralph Vandiest

Hello,on beech bark I found these orange/red peri

18-09-2013 19:19

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Good evening,I cannot determinate this small Peziz

19-09-2013 12:20

Gilles Corriol Gilles Corriol

Bonjour à tous, Mon dernier Mollisia n'a eu aucu

18-09-2013 16:37

Ivana Kusan

Hi to all!Can someone help me with the following t

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Claussenomyces sp. with reddish brown pigments
Ethan Crenson, 21-10-2018 23:44
Hello all,

Yesterday in Eastern New Jersey (US), I found a number of very small black discs on decorticated wood. The are about .5 mm in diameter, some smaller, one approaches 1mm.  Microscopically they remind me of Classenomyces, with spores that appear at first fusoid and single septate.  They then develop multiple septations (5-6 septate) with vertical septations in the center cells.  Finally they appear to break apart into tiny (perhaps globose, hard to see) part spores.  Asci are around 100-136 by 12-13 µm.   Ascospores—the most mature multi-septate ones—measured in ascus were around 16-21 by 7-8 µm.  Paraphyses were surrounded by brownish red material.  Application of KOH appeared to dissolve most of it.

I tried the key "Provisionary key to Claussenomyces s. auct. (G. Marson + H.O. Baral 17. März, 1994, improved 14.09.1996 and 2002)" which suggested C. canariensis ... but perhaps I am not using it correctly.  

Any ideas on this?

Thank you in advance.
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Hans-Otto Baral, 22-10-2018 09:13
Hans-Otto Baral
Re : Claussenomyces sp. with reddish brown pigments
Hi Ethan
this is an interesting fungus. I assume that the asci are inamyloid in Lugol (did you test it?). Also the dissolution of the brown pigment probably causes a short-lasting brown stin to the medium (ionomidotic). 

It could be a member of Cordieritidaceae, and here in the genus Skyttea, but I do not remember muriform spores in this family. Clearly the spores of your fungus get muriform within the living asci.

But there are other families with the same KOH reaction. I think our Claussenomyces-key, in which the ionomidotic reaction rarely occurs, is not the right place to identify this fungus.

Zotto
Ethan Crenson, 23-10-2018 17:14
Re : Claussenomyces sp. with reddish brown pigments
Zotto,

It is inamyloid in Lugol's solution.  I tried applying KOH directly to the fruiting body to attempt to see the ionomidotic reaction, but unfortunately I got very little pigment, a very faint reaction if any.  Perhaps my interpretation of the previous mount was in error. 

Ethan
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Hans-Otto Baral, 23-10-2018 17:37
Hans-Otto Baral
Re : Claussenomyces sp. with reddish brown pigments
If you looked immediately after placing the apo in KOH then it is actually weak. Another possibility is to add KOH to the water preparation and take a pohto in the first seconds when the KOH reaches the tissue.